LOCUS       sequence-94318-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged Human PLC delta PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  TITLE     Levine plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRS414-7x2-PHO5-GFP-hPLC delta PH domain dimer
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(15..33)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    74..577
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        614..718
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgcgcgagcg aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
       61 ggtttcttag acggatcgct tgcctgtaac ttacacgcgc ctcgtatctt ttaatgatgg
      121 aataatttgg gaatttactc tgtgtttatt tatttttatg ttttgtattt ggattttaga
      181 aagtaaataa agaaggtaga agagttacgg aatgaagaaa aaaaaataaa caaaggttta
      241 aaaaatttca acaaaaagcg tactttacat atatatttat tagacaagaa aagcagatta
      301 aatagatata cattcgatta acgataagta aaatgtaaaa tcacaggatt ttcgtgtgtg
      361 gtcttctaca cagacaagat gaaacaattc ggcattaata cctgagagca ggaagagcaa
      421 gataaaaggt agtatttgtt ggcgatcccc ctagagtctt ttacatcttc ggaaaacaaa
      481 aactattttt tctttaattt ctttttttac tttctatttt taatttatat atttatatta
      541 aaaaatttaa attataatta tttttatagc acgagatgaa aaggacccag gtggcacttt
      601 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      661 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      721 gagtattcaa catttccgtg tcgcccttaa ttccc
//
