LOCUS       sequence-94321-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged OSBP PH domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Levine TP, Munro S
  TITLE     Targeting of Golgi-specific pleckstrin homology domains involves 
            both PtdIns 4-kinase-dependent and -independent components.
  JOURNAL   Curr Biol. 2002 Apr 30;12(9):695-704.
  PUBMED    12007412
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2002-04-30"; volume: "12(9)"; pages: "695-704"
COMMENT     Sequence Label: pBGPa-CMV-GFP-OSBP PH domain
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..43
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(407..426)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    451..499
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gcttggatcc atggagcaaa agctcatttc tgaagaggac ttgagatctt cgggctcggc
       61 tcgagagggc tggctcttca aatggaccaa ttatatcaaa ggctaccagc ggcgatggtt
      121 cgtgctgagc aacgggctcc tgagctacta cagatcaaag gcagagatga gacatacctg
      181 ccgtggtacc atcaacctcg ccacagccaa catcaccgtg gaggactcct gcaacttcat
      241 catttccaat gggggtgctc agacctacca tctgaaagct agttcagaag ttgagcggca
      301 gcgctgggtg acggccctgg aactggccaa ggccaaagct gtgaagaaga attcgtgata
      361 accgcggtgg agctctagag atccccggga gatgggggag gctaactgaa acacggaagg
      421 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
      481 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
      541 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
      601 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
      661 atagccactg gccccgtggg ttagggacgg ggtcccccat gctagctttt tgc
//
