LOCUS       sequence-94323-e       10995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  drives ZIF-1 protein in C. elegans neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Armenti ST, Lohmer LL, Sherwood DR, Nance J
  TITLE     Repurposing an endogenous degradation system for rapid and targeted 
            depletion of C. elegans proteins.
  JOURNAL   Development. 2014 Dec;141(23):4640-7. doi: 10.1242/dev.115048. Epub 
            2014 Nov 5.
  PUBMED    25377555
REFERENCE   2  (bases 1 to 10995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.115048"; journalName: "Development"; date: "2014-12"; 
            volume: "141"; issue: "23"; pages: "4640-7"
COMMENT     Sequence Label: pSA101
FEATURES             Location/Qualifiers
     source          1..10995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3661..3678)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3661..3677)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5385..5404)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5504..5526
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5564..5582)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5650..5754
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5755..5823,5824..6615)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5973..5992)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6786..7374
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7275..7294
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9010..9021)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atcttcagat gggagcagtg gactgtctat ttgatattct taaaatgtac tctataaaag
       61 atgaaactgt aatgaccaat tccctacata aatattttta actaattggt cggatatttg
      121 gggatcagag aagtacttgt ttttgcgata ttaataatga tatatttttc tagtaaaaaa
      181 atgttaagcg atttgtgaat aattaagttg gcttgaaaaa gattttttga tagctatgta
      241 ttttgttaat ttaaatcaga ttttaaaact ttattattga ctatttatat atattatata
      301 ttatatattt tcatggttta atcaaaatct attaaattat ttctaaaatc tctaaatatc
      361 gaattctcta aaaagcttca tcaacaaaag catttcaatt atctaacaca atgagaataa
      421 ttcaagaact gaaatagaaa gaaatctaga aaaaaaagag agtgaggtgc cctattaaat
      481 cggtgctctc ctctctaaca gaagcgccta cacgcaccca agagagctct ttttctttct
      541 tttttcggag cacacataca cgcatacact cctcagaagt gtatagagaa aatgggagaa
      601 gaagcgaaag aagacgaaga agcggttgtg atgtatgcat cgggcgtttc tatctctctc
      661 cgtgagcaac gagctagtca acccatcgat ccatttttgt gagcacacac agagagagac
      721 tcaaacacac tcacagcttc acactgagct ccaagagctc tgtagagaga agccagagta
      781 tgctttcctc gattttccta acgtcttctt cttctgcttt tttctttttc ccaacgagtt
      841 gtagtccact aacttcaatt tttcctccag agaaagagtt ttagagagac agctgataaa
      901 tacactagag aaataaaaag aatgataaaa atttgctatt ttaatcagtt ccctctcgtt
      961 tctctaagtt ttgttgttgg gttgggaaat taaaattgga aaatggaaat aacgtttttt
     1021 tctgtctaat cttgaattct ccagacattt cttatttcct tttttgttgc aagtttttct
     1081 tccttctcta atttttaatc aaagatatct cgctatgaac tttgaatttt tgatattttg
     1141 attctataaa tacataaagt aatctaaatg ctcttttaaa ataaatctac agtagcccta
     1201 ttttcagatg agtgagtgtt ccgcgagtac cagtcagtta tctactttct gtgtaaatga
     1261 gataccatgg aagttggttc cacaggtaaa aatcttgaaa accactcagt ttcgacaaaa
     1321 caaaaatttc agacttgcga cgaattcagc gaatacgagt tcatagatga aaaatatagg
     1381 tgaggatctc tagatatata tctattaaat tttataacct tttcaggcgc ctttctgacg
     1441 tagaatcgtt tgtctggaag tggtcgccgt gtggaagata tggaattgat cttccgggcg
     1501 gtcgtgacgt cattaaccaa caactacgtg accgtatggc tgtattcgat ctacatacga
     1561 acaaatggac cctatacacc atggaccgtg gtgctttcga aattccacga gtttgctttc
     1621 tctactgggc acgaaacgat ataatcggca caataaccct tgtagtaagt gtccgatcta
     1681 ccatacctaa tccaaaaaat aaaatttcag aaagactatg aagcaggacc ggatgatgaa
     1741 attccattga aattcaagca atcattcttc tacgtatctc acgaaacaca gacattagtg
     1801 tactctggtt catgcaagta ccgtacgaat aaaacaatcg tagcatctta ttggtaccat
     1861 attttcgaaa atcgacgtgg cgaaccgttc attgatcaca ataactacct gtggttggta
     1921 ttctcaaacg gagtggattc tctgattctt cttccattta ttccaacttc aacgtgtttc
     1981 aaagcgaaat gtgtatcatt tgatatgaac gatatcgtca gtcaattcac tggtattcgc
     2041 gtatttctcg atccatttgt atcatctcac tacgtgccat gggtctacag aaatgacgtt
     2101 aattttttca ttaggattga ttcgcattca tttatcgagg agcaaaaccg ggactatggt
     2161 ctagatccgc aaattgataa cccggagacg aaaccgcaca gaggagtatt tcaacttcgt
     2221 gtgaatttga aagatgtcat cgaaaagggc gaaaaagatg cgattcatgc agttaatcct
     2281 gaaacaatag agacaagatt caatcgaggt gacattgaac tatggcgaaa aatcagcgat
     2341 gagctccgtc aagaacatca gagcatagca cagcatggca aaatggttgt agtacaacga
     2401 tggtcacact ttactgttga tatggggctc agcaagaaaa tgagaaccgt aggatatgaa
     2461 aatattattt gttgatagtt tattaattta atttcagcgt gttgccgatt tggacgcccg
     2521 tgattactgt tgcagccata agccacgtaa ttcgtcattc tgctgtattg acttggacag
     2581 cgagcgaatc aaggctgtgg atgctctcat tcttgggtac aaattaatct atttttttcc
     2641 taagtatttt tttttcagaa ctgacgtggt tcttccccat cctagtggct ctgtattcct
     2701 atttcgatac aaggaaaatt atggtaatcc ctccaccaaa tagacgtttt cttcatcgta
     2761 atttttcagg gatgagttat tgcgagcttc cattcttcca gccactttca ttgaagctaa
     2821 aatgtattca agttctcaag gaatctgtcg aaatcgaccc aagttactat agaatcaaat
     2881 gctgccttaa aatgcaagaa gaacttgaaa aagaaactgc cgaaaatttg tgcacgataa
     2941 atattcaaca ataagtccaa ttactcttca acatccctac atgctctttc tccctgtgct
     3001 cccaccccct atttttgtta ttatcaaaaa aacttcttct taatttcttt gttttttagc
     3061 ttcttttaag tcacctctaa caatgaaatt gtgtagattc aaaaatagaa ttaattcgta
     3121 ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct atcccaaaat
     3181 ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt ttttttgaaa
     3241 catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc
     3301 gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga
     3361 aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt
     3421 cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt
     3481 tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg
     3541 gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat
     3601 ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttaaac
     3661 actggccgtc gttttacact cgagacgtac ggtgcgcgcg atgcattcga agatctgccc
     3721 actagtgagt cgtattataa gtgcaagtaa gatcagtgtt tgtttcgata tcagtctgtt
     3781 tcgtaacggt cttctgtata actacaaaaa agaataaaaa accgtatcac aactgagaaa
     3841 aaagacaaaa cttattgaac agtgttcctt tcaaaaaatt attttgtaaa aaatcgagtt
     3901 tttctccaaa ataacgcacg caatcaaaaa caagaaaact aacgtgttct cctgggcgtg
     3961 gaactcctta tcaagattac cattattgtc tgtcagaatt gatatttcaa ttatttcata
     4021 tctagacaaa cccgggtgtc ttgcatttat tagtactgtg agtttacata caaacgaggc
     4081 ttactcagat aacatagagt cacctctaat tagcaaaaac ccatttctaa ttagctattt
     4141 cgaccagttc taaagtcacc gtaattagaa atggtttttg tagcttttct aattagaaat
     4201 gaccccgaga taagtgaaat atggcatttt ctgggttttc tagtaagaaa tgatcccgca
     4261 cgtagtgatt ccgcgttaca agccaaattc aaattaaaaa agtatattac acaattcaag
     4321 ttacattatt ttcaggaaac aatggcgaag agcaaagcag agattcaaaa ggaggaaaga
     4381 gacaacagat atgaacgaag acttcttgaa aagagaaaga gacatgaggt gagtaaccat
     4441 gataataaat aatcaagtaa gggctataga tttataaatt ctggagttaa cagtttcgaa
     4501 aaaaagtagt tttcatatgt gcgaaggaat atcaaaaata ggggaatagc ctaaacgagt
     4561 gagaaaatta aatgtttgtt attttttcaa aaatgtctgc agggccgtta tcctttcaaa
     4621 tgcactctat caaacataga tcatatccat catatcttat ccagaattta tatcatatcc
     4681 aagattgaga gcgtttcaag gaaaatctca aacaaccata caattttgta gatcgacaac
     4741 aaggcctcca cttcttcatc gatggcctct gcagcacctg caccaccaag tttgtatttt
     4801 tcatattaat ataaagtgag ccacgcatag tcgtttttag gatttttgcc agatcgatgt
     4861 cgcgcaaaca gactgttttt ttttctttga aagccgtgcg tgacatgggc cacggattgt
     4921 aacgccgcgc tggatggata aaaatttatt cagtttttta gcctttacaa aatatggttc
     4981 gactactttc tttattttta catttttata ggtgctcatt gagctgagat attttgggta
     5041 taatcggaaa atcgctgatt ttggtcattt ttctgcacta aagattacac aaaaagttca
     5101 gtatttagat tcattgttga aaattgggga tgatctacag atacatatcg ccgtgttcaa
     5161 aaacacacgt gctgtgacgt cgtgactggg gaaaaccctg gcgttaccca acttaatcgc
     5221 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     5281 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     5341 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     5401 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     5461 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     5521 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     5581 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     5641 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5701 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5761 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5821 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5881 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5941 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     6001 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6061 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6121 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6181 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6241 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     6301 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6361 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6421 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6481 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6541 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6601 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6661 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6721 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6781 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6841 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6901 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     6961 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7021 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7081 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7141 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7201 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7261 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7321 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7381 agcaacgcgg cctttttacg gttcctggtc ttttgctggc cttttgctca catgttcttt
     7441 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7501 agcggataac aatttcacag ggccctgtcg gattatgggc tcttctttta tcctctactt
     7561 tctttctaga aaatgcaaat gttgagtcag aaattgctga atggagtaca actgcaatgg
     7621 gtttagctta tgtttctgga acaattactg gtggaattgt gattgaaaag taaggttata
     7681 tttcatttca ataaatgtta ccacagtata acaaaaacaa gttacattgt tcagagtatg
     7741 tctgaatgta ttattattat tgtatttgtg catcagtact gtagttgtac tgaagaaggg
     7801 tttaaaataa tctcaacgtt tttaagacga gttagtgaga atacggtcgc agttaggatg
     7861 tttggacata gttttattag ataatcgtga actaactcac tataaaaacc cgctaactca
     7921 ttgaataaga tagttttaaa cgttcaaaat ttgaagacaa aattgtatga ataagtttac
     7981 agagttggtc gacgcaagct tctccttctc ttcacatttc tcaataattt agctctaatt
     8041 gcattcgtat tcttcgcaaa aatacgtatt ctgattgacc cgatgaaata cggatgccta
     8101 gttgcattga tcatctacgg ttatacttat gggtgaggtt tttcaaatat attttgattt
     8161 aatcgaataa tgaaatttca gaactggagt tggtccaatt tcgtggttta tatcctctga
     8221 actggtacct caaaaacatc gtagtattgc tcaatctgtg gcatattcaa tgaatactgt
     8281 aatggtggtt atcagtacat ttacagtatt acctctatat tccgttattg gtagttatgc
     8341 tttccttatt ctatattcaa ttccttcatt tataagtatg cttatattat ttcgctactt
     8401 accggaaacc aaaggacgag agattcatga tattgtgaat gaacttaaaa aaaaatgaaa
     8461 acctttgaaa acgttcatac ggccgaaatt ttacttgatc gagccaattg aacttttgtt
     8521 aagacttaca gaaaagtgag agattttgtt acaaaaaagt gagaaaaaag cgaaaatgaa
     8581 caggaaaaag aagaaaaata cacccgaagt atcatataaa aattaaaaag attgtttgac
     8641 ctggcggaac ttttttgcaa aaactctgaa tatccatggc acatgcttcg tgcaaaacag
     8701 gatccaaatg aatatcaaca agagattctt gaagacgacg agccacttgc tcagagcatt
     8761 gcttatctcg aatgagaccc ttgttaaaat catttttcaa acattatatg acattgtcga
     8821 aatgtcctcc tgattccatg atggtagcaa actcacttcg tctttgagcc aatttatcca
     8881 agtccttgta aaaagtattc gaaaattgtt aacggataaa atgtttatta taatatcaaa
     8941 aacaatttgt cagttgacca ctttttgata tatttgacag aaaccgggat gaattggctc
     9001 aaagttaggg cgcccttcta ttacagggtt tctgataaca aaccggttat taactcccaa
     9061 caagggatga tttcaattca tcatgctcaa attgacccaa attaagttac atgacaaatt
     9121 catcgccctt ttcaactctt cttggctcat catctgttat tctgttccta ttctgtctgc
     9181 accctatacc cctttgcata ctctctcgtc attcctttct tggatagtgt cttcttctcc
     9241 ccagctctgc tacttctatg acttgcgccc gctgcttttc cgctcgttcc tctcctctct
     9301 gacgtcttct tctgcttctt ttctccttcc tttttccctt ctcaatcttc ctctttttcc
     9361 catttctgtc aatcattcga agaagaagaa gaagaagacc ctcattcatt tcattttttt
     9421 ttctgtcggt gtgtgctgct cggttaagag tgagctctct attccacgtc ttcttctttt
     9481 tcttcttcga ttcgaatcaa tcactccacc aaaacgcatt cgtttttggg attcaccccg
     9541 cggttcgcaa taggtttctt tttcaaatta ttagcgttat aaatagaaaa atggtggagt
     9601 ttcaataaaa atgataattt acaaagtgta ttttgattac atgtactcaa aaaggttgaa
     9661 atttttcata ccagttttcc ggaaatccat ctgatatcat tatcgtattt ttctttttaa
     9721 aaatgttttc aaaaaaaaac aaaatatagc tggttatttg gcaccctcta attaccattt
     9781 tcctgtcaca ccacactctt ttccttcttc cacttctttc cgttttcagc cgcttccaac
     9841 caaaccgata tgaaagccga gcaacaacga tcgattccac ccggctcggc gaccttcccg
     9901 tcgcaggtga gactcagaaa actagagaaa ccgctcaact aactcttgat atccgatttc
     9961 attcttttct cttttctttt tgttgaactt ttccatattt ccagatgcca cggccaccac
    10021 caagcaccga acaaggaatc acaacggaat cggagcttgc gaagaaagct caaatcactc
    10081 cgaacgacgt tttagcactt ccgggaatca ctcaaggtat cttgctccct ttggatttca
    10141 gtttaaaaca taaatttagg attcttatgc tccccatctg cgaacatcta taacatcgag
    10201 ttcaccaagt tccaaatccg tgatctggac actgagcaag tgcttttcga gatcgccaaa
    10261 ccggagaacg atcaggagaa tgatgagtcg ccacaggagt cggcaagata cgtgcgttat
    10321 agatttgctc caaacttttt gaaactcaaa acggtcggag caactgtgga attcaaagta
    10381 ggagacatcc caatccatca tttccgaatg atcgaacgtc acttcttcaa agatcgcctt
    10441 ctgaagtgtt ttgactttga attcggattc tgtattccga attcacgaaa caactgtgaa
    10501 catatctatg agttccctca actctctcaa caactcagtg agtcattatt ctaaaaagta
    10561 caattcaaaa gactaatctc tttttcagtg gacgacatga tcaacaatcc aaacgagact
    10621 cgttctgaca gcttctattt cgtcgataac aaactcgtca tgcacaacaa agccgactac
    10681 tcatatgatg cataaatatt taatacaaaa atgttctgga taattattct gtcgaataga
    10741 aaaaaaactc caaatgtgat taaattccaa taattcctgt ctagtttgtt ccttcccctt
    10801 ccccttctca tgttcaatgc attcctaagc ttttcagttc cccccttgtt tctatatttt
    10861 ttcgctgtcc tgtcacactc gctaaaaaca ctaatcacac ggaaatctgt tttcaataaa
    10921 aactccaact ttaactcatt ttcaatttca actgaaagat tttttcatta gagaatgtct
    10981 agaactaggc cgttt
//
