LOCUS       sequence-94324-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged CERT PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Levine TP, Munro S
  TITLE     Targeting of Golgi-specific pleckstrin homology domains involves 
            both PtdIns 4-kinase-dependent and -independent components.
  JOURNAL   Curr Biol. 2002 Apr 30;12(9):695-704.
  PUBMED    12007412
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2002-04-30"; volume: "12(9)"; pages: "695-704"
COMMENT     Sequence Label: pRS406-PHO5-GFP-CERT PH domain
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..54
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(459..479)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(459..477)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(498..514)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(498..514)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(511..533)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    522..538
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(546..552,553..570,571..576))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    591..612
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(729..746)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agactataca agcttggatc catggagcaa aagctcattt ctgaagagga cttgagatct
       61 atgtcggata atcagagctg gaactcgtcg ggctcggagg aggatccaga gacggagtct
      121 gggccgcctg tggagcgctg cggggtcctc agtaagtgga caaactacat tcatgggtgg
      181 caggatcgtt gggtagtttt gaaaaataat gctctgagtt actacaaatc tgaagatgaa
      241 acagagtatg gctgcagagg atccatctgt cttagcaagg ctgtcatcac acctcacgat
      301 tttgatgaat gtcgatttga tattagtgta aatgatagtg tttggtatct tcgtgctcag
      361 gatccagatc atagacagca atggatagat gccattgaac agcacaagac tgaatctgga
      421 tatggatgaa tctagagatc cccctccagc ttttgttccc tttagtgagg gttaattgcg
      481 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      541 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      601 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      661 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
      721 tccgcttcct cgctcactga ctcgctgcgc tcgggtcgtt cggctgcggc gagcggtatc
      781 agctcactca aaggcggtaa tacggttatc cacag
//
