LOCUS       sequence-94327-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged CERT PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Levine TP, Munro S
  TITLE     Targeting of Golgi-specific pleckstrin homology domains involves 
            both PtdIns 4-kinase-dependent and -independent components.
  JOURNAL   Curr Biol. 2002 Apr 30;12(9):695-704.
  PUBMED    12007412
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2002-04-30"; volume: "12(9)"; pages: "695-704"
COMMENT     Sequence Label: pRS406-PHO5-GFP-CERT PH domain
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     231..250
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(545..573)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacgct ctcccttatg cgactcctgc
       61 attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt
      121 gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
      181 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
      241 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
      301 gcgtagagga tcgatccggt caccttactt ggcaaggcat atacccattt gggataaggg
      361 taaacatctt tgaattgtcg aaatgaaacg tatataagcg ctgatgtttt gctaagtcga
      421 ggttagtatg gcttcatctc tcatgagaat aagaacaaca acaaatagag caagcaaatt
      481 cgagaattcc tgcagcccgg gcaattcgaa tataatgtct aaaggagaag aacttttcac
      541 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      601 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
      661 tactggaaaa ctacctgttc catggccaac acttgtcact acttttacgt atggtgttca
      721 atgcttttca agatacccag atcatatgaa acggcatgac tttttcaaga gtgccatgcc
      781 cgaaggttat gtacaggaaa gaactata
//
