LOCUS       sequence-94330-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged Osh2 PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Roy A, Levine TP
  TITLE     Multiple pools of phosphatidylinositol 4-phosphate detected using 
            the pleckstrin homology domain of Osh2p.
  JOURNAL   J Biol Chem. 2004 Oct 22;279(43):44683-9. Epub 2004 Jul 22.
  PUBMED    15271978
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-22"; volume: "279(43)"; pages: "44683-9. Epub
            2004 Jul 22"
COMMENT     Sequence Label: pRS406-PHO5-GFP-2xOsh2 PH domain [short]
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..29
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11..29
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(734..763)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gccagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggttatc
       61 acgaattctt gtctttggcg aatctaatag cgctttgtat agcattgacc cacctagtcg
      121 tttcaatggg atgattgccc tttaagcgcc accttgttgt gcctgtgatg ccacctagca
      181 actcgaaatt cagtttttct gaagaatcga tgtgtaagcg gcacgtcgaa acttttaaag
      241 taccacgagg cctatcaaca tgcgattggt ctttgtaata tgacagattt ccgtcgccac
      301 ttaaaatgaa ccaacgtaat ttgtagccat gggcaaaatt tgtccatttc tttaaaaacc
      361 ccttatacgt aggaggctta cttgaagatc cggcaccagc accggcgccc ttgtctttgg
      421 cgaatctaat agcgctttgt atagcattga cccacctagt cgtttcaatg ggatgattgc
      481 cctttaagcg ccaccttgtt gtgcctgtga tgccacctag caactcgaaa ttcagttttt
      541 ctgaagaatc gatgtgtaag cggcacgtcg aaacttttaa agtaccacga ggcctatcaa
      601 catgcgattg gtctttgtaa tatgacagat ttccgtcgcc acttaaaatg aaccaacgta
      661 atttgtagcc atgggcaaaa tttgtccatt tctttaaaaa ccccttatac gtaggaggct
      721 tacttgaaga tctcaagtcc tcttcagaaa tgagcttttg ctccatggat ccaagcttgt
      781 atagttcatc catgccatgt gtaatcccag cagctgttac aaactc
//
