LOCUS       sequence-94333-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 588, Emission:
            635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, 
            Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, 
            Chudakov DM
  TITLE     Bright far-red fluorescent protein for whole-body imaging.
  JOURNAL   Nat Methods. 2007 Sep . 4(9):741-6.
  PUBMED    17721542
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Sep . 4(9):741-6."
COMMENT     Sequence Label: mKate-pBAD
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..731)
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             complement(738..758)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(771..803)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(807..824)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agccaagctt cgaattctca attaagtttg tgccccagtt tgctagggag gtcgcagtat
       61 ctggccacag ccacctcgtg ctgctcgacg taggtctctt tgtcggcctc cttgattctt
      121 tccagtcttc tgtccacata gtagacgccg ggcatcttga ggttcttagc gggtttcttg
      181 gatctgtatg tggtcttcaa gttgcagatc aggtggcccc cgcccacgag cttcagggcc
      241 atgtcgcttc tgccttccag gccgccgtca gcggggtaca gcatctcggt ggaggcctcc
      301 cagccgagtg ttttcttctg catcacaggg ccgttggatg ggaagttcac ccctctgatc
      361 ttgacgttgt agatgaggca gccgtcctgg aggctggtgt cctgggtagc ggtcagcacg
      421 cccccgtctt cgtatgtggt gactctctcc catgtgaagc cctcagggaa ggactgctta
      481 aagaagtcgg ggatgccctg ggtgtggttg atgaaggttt tgctgccgta catgaagctg
      541 gtagccagga tgtcgaaggc gaaggggaga gggccgccct cgaccacctt gattctcatg
      601 gtctgggtgc cctcgtaggg cttgccttcg ccctcggatg tgcacttgaa gtggtggttg
      661 ttcacggtgc cctccatgta cagcttcatg tgcatgttct ccttaatcag ctcttcgccc
      721 ttagacacca tgctcgagcc ctggaagtac aggttctcgt acagatcccg acccatttgc
      781 tgtccaccag tcatgctagc cataccatga tgatgatgat gatgagaacc c
//
