LOCUS       sequence-94335-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 588, Emission:
            635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, 
            Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, 
            Chudakov DM
  TITLE     Bright far-red fluorescent protein for whole-body imaging.
  JOURNAL   Nat Methods. 2007 Sep . 4(9):741-6.
  PUBMED    17721542
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Sep . 4(9):741-6."
COMMENT     Sequence Label: dKatushka-pBAD
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..720)
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHSQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             complement(727..747)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(760..792)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(796..813)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcttcgaat tctcagctgt gccccagttt gctaggcagg tcgcagtacc tggccacagc
       61 catctcgtgc tgctcgacgt aggtctcttt gtcggcctcc ttgattcttt ccagtctcct
      121 gtccacgaag tagaagccgg gcatcttgag gttcttagcg ggtttcttgg atctgtatgt
      181 ggtcttgagg gagcagtgca ggtagccccc gcccacgagc ttcagggcca tctggctatg
      241 gcctctcagg ccgctgtcag cggggtacag catctcggtg ctggcctccc agccgagtgt
      301 tttcttctgc atcacagggc cgttggatgg gaagttcacc ccgttgatct tgacgttgta
      361 gatgaggcag ccgttctgga ggctggtgtc ctgggtagcg gtcagcacgc ccccgtcttc
      421 gtatgtggtg atcctctccc atgtgaagcc ctcagggaag gactgcttaa agaagtcggg
      481 gatgccctgg gtgtggttga taaaggtttt gctgccgtac atgaagctgg tagccaggat
      541 gtcgaaggcg aaggggagag ggccgccctc gaccaccttg atcttcatgg tctgggtgcc
      601 ctcgtagggc ttgccttcgc cctcggatgt gcacttgaag tggtggtcgt tcacggtgcc
      661 ctccatgtac agtttcatgt gcatgttctc ggtgatcagc acgctatcct cacccaccat
      721 gctcgagccc tggaagtaca ggttctcgta cagatcccga cccatttgct gtccaccagt
      781 catgctagcc ataccatgat gatgatgatg atgagaaccc cgcatatgta tatctccttc
      841 ttaaagttaa
//
