LOCUS       sequence-94338-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged Osh2 PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Roy A, Levine TP
  TITLE     Multiple pools of phosphatidylinositol 4-phosphate detected using 
            the pleckstrin homology domain of Osh2p.
  JOURNAL   J Biol Chem. 2004 Oct 22;279(43):44683-9. Epub 2004 Jul 22.
  PUBMED    15271978
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-22"; volume: "279(43)"; pages: "44683-9. Epub
            2004 Jul 22"
COMMENT     Sequence Label: pRS406-PHO5-GFP-2xOsh2 PH domain [short]
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(704..724)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(704..722)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(743..759)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(743..759)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(756..778)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 atcttcaagt aagcctccta cgtataaggg gtttttaaag aaatggacaa attttgccca
       61 tggctacaaa ttacgttggt tcattttaag tggcgacgga aatctgtcat attacaaaga
      121 ccaatcgcat gttgataggc ctcgtggtac tttaaaagtt tcgacgtgcc gcttacacat
      181 cgattcttca gaaaaactga atttcgagtt gctaggtggc atcacaggca caacaaggtg
      241 gcgcttaaag ggcaatcatc ccattgaaac gactaggtgg gtcaatgcta tacaaagcgc
      301 tattagattc gccaaagaca agggcgccgg tgctggtgcc ggatcttcaa gtaagcctcc
      361 tacgtataag gggtttttaa agaaatggac aaattttgcc catggctaca aattacgttg
      421 gttcatttta agtggcgacg gaaatctgtc atattacaaa gaccaatcgc atgttgatag
      481 gcctcgtggt actttaaaag tttcgacgtg ccgcttacac atcgattctt cagaaaaact
      541 gaatttcgag ttgctaggtg gcatcacagg cacaacaagg tggcgcttaa agggcaatca
      601 tcccattgaa acgactaggt gggtcaatgc tatacaaagc gctattagat tcgccaaaga
      661 caagaattcg tgataaccgc ggtggagctc cagctttttg ttccctttag tgagggttaa
      721 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      781 c
//
