LOCUS       sequence-94342-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged and mutated CERT PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Roy A, Levine TP
  TITLE     Multiple pools of phosphatidylinositol 4-phosphate detected using 
            the pleckstrin homology domain of Osh2p.
  JOURNAL   J Biol Chem. 2004 Oct 22;279(43):44683-9. Epub 2004 Jul 22.
  PUBMED    15271978
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-22"; volume: "279(43)"; pages: "44683-9. Epub
            2004 Jul 22"
COMMENT     Sequence Label: pRS406-PHO5-GFP-CERT PH domain EE mutation
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(3..21)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        89..193
                     /gene="bla"
                     /label=AmpR promoter
     CDS             378..756
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(412..431)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
       61 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      121 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      181 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      241 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      301 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      361 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      421 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      481 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
      541 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      601 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
      661 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
      721 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
      781 acttactcta gc
//
