LOCUS       sequence-94349-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged human FAPP1 PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Levine TP, Munro S
  TITLE     Targeting of Golgi-specific pleckstrin homology domains involves 
            both PtdIns 4-kinase-dependent and -independent components.
  JOURNAL   Curr Biol. 2002 Apr 30;12(9):695-704.
  PUBMED    12007412
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2002-04-30"; volume: "12(9)"; pages: "695-704"
COMMENT     Sequence Label: pRS406-PHO5-GFP-hFAPP1 PH domain
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..29
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11..29
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(362..391)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(444..464)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gccagcgcgc aattaaccct cactaaaggg aacaaaagct ggagggggat ctctagaatc
       61 atgtatcagt caaacatgct ttggagctcc ccagagcgac cagccacctc tgtctttcag
      121 ctgcattcac tgccttcatg tagaaatgct gctctccagg aatgattaat tccattcttg
      181 tgttgtctgc tgaatgaact ttaatttcac aaactgccat ctttatgctt cctttgctcc
      241 ctttgcaaac atcatcttgt gaatcatagt aggataagat tccattatct aaaacaaacc
      301 aacgaggctg ccagcctgtg agatagttgg tccacttgta caacacaccc tccatagatc
      361 tcaagtcctc ttcagaaatg agcttttgct ccatggatcc aagcttgtat agttcatcca
      421 tgccatgtgt aatcccagca gctgttacaa actcaagaag gaccatgtgg tctctctttt
      481 cgttgggatc tttcgaaagg gcagattgtg tggacaggta atggttgtct ggtaaaagga
      541 cagggccatc gccaattgga gtattttgtt gataatggtc tgctagttga acgcttccat
      601 cttcaatgtt gtgtctaatt ttgaagttaa ctttgattcc attcttttgt ttgtctgcca
      661 tgatgtatac attgtgtgag ttatagttgt attccaattt gtgtccaaga atgtttccat
      721 cttctttaaa atcaatacct tttaactcga ttctattaac aagggtatca ccttcaaact
      781 tgacttcagc acgtgtcttg tagttcccgt catctttg
//
