LOCUS       sequence-94351-e         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged human FAPP1 PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Levine TP, Munro S
  TITLE     Targeting of Golgi-specific pleckstrin homology domains involves 
            both PtdIns 4-kinase-dependent and -independent components.
  JOURNAL   Curr Biol. 2002 Apr 30;12(9):695-704.
  PUBMED    12007412
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2002-04-30"; volume: "12(9)"; pages: "695-704"
COMMENT     Sequence Label: pRS406-PHO5-GFP-hFAPP1 PH domain
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(342..362)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(342..360)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(381..397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(381..397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(394..416)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    405..421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(429..435,436..453,454..459))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    474..495
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(612..629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaggacttga gatctatgga gggtgtgttg tacaagtgga ccaactatct cacaggctgg
       61 cagcctcgtt ggtttgtttt agataatgga atcttatcct actatgattc acaagatgat
      121 gtttgcaaag ggagcaaagg aagcataaag atggcagttt gtgaaattaa agttcattca
      181 gcagacaaca caagaatgga attaatcatt cctggagagc agcatttcta catgaaggca
      241 gtgaatgcag ctgaaagaca gaggtggctg gtcgctctgg ggagctccaa agcatgtttg
      301 actgatacat gattctagag atccccctcc agcttttgtt ccctttagtg agggttaatt
      361 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      421 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      481 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      541 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      601 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      661 tcagctcact caaaggcggt aatacggtta tccacagaat ca
//
