LOCUS       sequence-94355-e         356 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Chen J, Mitcheson JS, Lin M, Sanguinetti MC
  TITLE     Functional roles of charged residues in the putative voltage sensor 
            of the HCN2 pacemaker channel.
  JOURNAL   J Biol Chem. 2000 Nov 17;275(46):36465-71.
  PUBMED    10962006
REFERENCE   2  (bases 1 to 356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2000-11-17"; volume: "275(46)"; pages: "36465-71"
COMMENT     Sequence Label: pSP64-mHCN2
FEATURES             Location/Qualifiers
     source          1..356
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        259..277
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     259..276
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     5'UTR           289..331
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat ctggctagcg atgaccctgc tgattggttc
       61 gctgaccatt tccgggtgcg ggacggcgtt accagaaact cagaaggttc gtccaaccaa
      121 accgactctg acggcagttt acgagagaga tgatagggtc tgcttcagta agccagatgc
      181 tacacaatta ggcttgtaca tattgtcgtt agaacgcggc tacaattaat acataacctt
      241 atgtatcata cacatacgat ttaggtgaca ctatagaata caagcttgct tgttcttttt
      301 gcagaagctc agaataaacg ctcaactttg gcatggatgc gcgcgggggc ggcggg
//
