LOCUS       sequence-94356-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-22"; volume: "9(7):e103084. doi"; pages: " 
            10.1371/journal.pone.0103084. eCollection 2014"
COMMENT     Sequence Label: 3xFLAG-dCas9/pMXs-neo
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(162..182)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcggccgctc gagtttaaat agtacataat ggatttcctt acgcgaaata cgggcagaca
       61 tggcctgccc ggttattatt atttttgaca ccagaccaac tggtaatggt agcgaccggc
      121 gctcagctgg aattcgatcc ggtagggatc gaaccctttc acaccttcct cttcttcttg
      181 gggtcagccc tgctgtctcc accgagctga gagaggtcga ttcttgtttc atagagcccc
      241 gtaattgact gatgaatcag tgtggcgtcc aggacctcct ttgtagaggt gtaccgcttt
      301 ctgtctatgg tggtgtcgaa gtacttgaag gctgcaggcg cgcccaagtt ggtcagagta
      361 aacaagtgga taatgttttc tgcctgctcc ctgatgggct tatccctgtg cttattgtaa
      421 gcagaaagca ccttatcgag gttagcgtcg gcgaggatca ctcttttgga gaattcgctt
      481 atttgctcga tgatctcatc aaggtagtgt ttgtgttgtt ccacgaacag ctgcttctgc
      541 tcattatctt cgggagaccc tttgagcttt tcatagtggc tggccagata caagaaatta
      601 acgtatttag agggcagtgc cagctcgtta cctttctgca gctcgcccgc actagcgagc
      661 attcgtttcc ggccgttttc aagctcaaag agagagtact tgggaagctt aatgatgagg
      721 tcttttttga cctctttata tcctttcgcc tcgagaaagt cgatggggtt tttttcgaag
      781 cttgatcgct ccatgattgt gatgcccagc agttccttga cgcttttgag ttttttagac
      841 ttccctttct ccactttggc cacaaccagt acactgtaag cgactgtagg a
//
