LOCUS       sequence-94357-e         259 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Chen J, Mitcheson JS, Lin M, Sanguinetti MC
  TITLE     Functional roles of charged residues in the putative voltage sensor 
            of the HCN2 pacemaker channel.
  JOURNAL   J Biol Chem. 2000 Nov 17;275(46):36465-71.
  PUBMED    10962006
REFERENCE   2  (bases 1 to 259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2000-11-17"; volume: "275(46)"; pages: "36465-71"
COMMENT     Sequence Label: pSP64-mHCN2
FEATURES             Location/Qualifiers
     source          1..259
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    79..100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(115..120,121..138,139..145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    153..169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     158..180
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     177..193
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     177..193
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
       61 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
      121 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      181 aaacagctat gacatgatta cgaattcgag ctcgcccggg gatcctctag agtcgacctg
      241 cagggggggg ggggggggg
//
