LOCUS       sequence-94361-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged OSBP PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Levine TP, Munro S
  TITLE     The pleckstrin homology domain of oxysterol-binding protein 
            recognises a determinant specific to Golgi membranes.
  JOURNAL   Curr Biol. 1998 Jun 18;8(13):729-39.
  PUBMED    9651677
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "1998-06-18"; volume: "8(13)"; pages: "729-39"
COMMENT     Sequence Label: pRS406-PHO5-GFP-OSBP PH domain
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..43
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(388..408)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(388..406)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(427..443)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(427..443)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(440..462)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    451..467
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(475..481,482..499,500..505))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    520..541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(658..675)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcttggatcc atggagcaaa agctcatttc tgaagaggac ttgagatctt cgggctcggc
       61 tcgagagggc tggctcttca aatggaccaa ttatatcaaa ggctaccagc ggcgatggtt
      121 cgtgctgagc aacgggctcc tgagctacta cagatcaaag gcagagatga gacatacctg
      181 ccgtggtacc atcaacctcg ccacagccaa catcaccgtg gaggactcct gcaacttcat
      241 catttccaat gggggtgctc agacctacca tctgaaagct agttcagaag ttgagcggca
      301 gcgctgggtg acggccctgg aactggccaa ggccaaagct gtgaagaaga attcgtgata
      361 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
      421 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      481 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      541 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      601 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      661 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      721 ggcggtaata cggttatcca cagaatca
//
