LOCUS       sequence-94363-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged Osh3 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Levine TP, Munro S
  TITLE     Dual targeting of Osh1p, a yeast homologue of oxysterol-binding 
            protein, to both the Golgi and the nucleus-vacuole junction.
  JOURNAL   Mol Biol Cell. 2001 Jun;12(6):1633-44.
  PUBMED    11408574
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2001-06"; volume: "12(6)"; pages: "1633-44"
COMMENT     Sequence Label: pRS416-PHO5-GFP-Osh3
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(6..24)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    65..568
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        605..710
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaagggcctc gtgatacgcc tatttttata ggttaatgtc atgatcataa tggtttctta
       61 gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg gaataatttg
      121 ggaatttact ctgtgtttat ttatttttat gttttgtatt tggattttag aaagtaaata
      181 aagaaggtag aagagttacg gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc
      241 aacaaaaagc gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat
      301 acattcgatt aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac
      361 acagacaaga tgaaacaatt cggcattaat acctgagagc aggaagagca agataaaagg
      421 tagtatttgt tggcgatccc cctagagtct tttacatctt cggaaaacaa aaactatttt
      481 ttctttaatt tcttttttta ctttctattt ttaatttata tatttatatt aaaaaattta
      541 aattataatt atttttatag cacgtgatga aaaggaccca ggtggcactt ttcggggaaa
      601 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      661 gagacaataa ccctgataaa tgcttccaat aatattgaaa aaggaagagt atgagtattc
      721 aacatttccg tgtcgccctt att
//
