LOCUS       sequence-94364-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Venkatachalam V, Cohen AE
  TITLE     Imaging GFP-Based Reporters in Neurons with Multiwavelength 
            Optogenetic Control.
  JOURNAL   Biophys J. 2014 Oct 7;107(7):1554-63. doi: 
            10.1016/j.bpj.2014.08.020.
  PUBMED    25296307
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2014.08"; journalName: "Biophys J"; date: "2014-10-7-
            7"; volume: "107"; issue: "7"; pages: "1554-63"
COMMENT     Sequence Label: VV246: CoChR(C108S)-GCaMP6f-ER in fck
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..66)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      78..87
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(94..108,109..117))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(121..153)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(157..174)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gttctcgagt gagctcagcc gacctatagc tctgactgcg tgacctgtct tattccactt
       61 acgacgtgat gagtcgacca tggtggcgag atccttatcg tcatcgtcgt acagatcccg
      121 acccatttgc tgtccaccag tcatgctagc cataccatga tgatgatgat gatgagaacc
      181 cattgctact accggtgccg ccactgtctc ctcgtcctcc tggtccacca tggtctccac
      241 ttccatctcc tcgccggcca cgttgatctt ggtcttcttc cggatgtcgc cgtagatgat
      301 gatgtgctgg tggatcagca ctctcagata gtgtcccagc aggccccaac aattcttgct
      361 catgaggtcg atgatggtgt ggccaatggt agagccgtac acgctgatgg cgtcgaatcc
      421 ttctggtccc acgacgaaca gcacggggaa catgccccaa gacaggaaga acagccaagc
      481 catgatccgc acgacggttc taggtctgcc ctttggcacc acgtggtagc tctcgatata
      541 caccttggcg gcgtggaaga aggtgttggc gccgtagatg caacccagca cgaagaagat
      601 gaccttgacg tagcctgtgc tcatggcgct tgtagctccc cacacgatgg ttcccacgtc
      661 tgacaccagc agcctcatgg tccgcttgct gtagtcgtcc ttcaggccgg tcaggttgct
      721 caggtggatc aggatgacgg gagaagtcag cagccactct gcgtatctca gccactggac
      781 tcggtgtccg ttagccaggt acagcatgct gg
//
