LOCUS       sequence-94365-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged Osh3 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Levine TP, Munro S
  TITLE     Dual targeting of Osh1p, a yeast homologue of oxysterol-binding 
            protein, to both the Golgi and the nucleus-vacuole junction.
  JOURNAL   Mol Biol Cell. 2001 Jun;12(6):1633-44.
  PUBMED    11408574
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2001-06"; volume: "12(6)"; pages: "1633-44"
COMMENT     Sequence Label: pRS416-PHO5-GFP-Osh3
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     249..268
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(563..591)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaagggaaca aaagctggag ctccaccgcg gtggcggccc cccctcgagg tcgacgctct
       61 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
      121 gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
      181 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
      241 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
      301 ccggccacga tgcgtccggc gtagaggatc gatccggtca ccttacttgg caaggcatat
      361 acccatttgg gataagggta aacatctttg aattgtcgaa atgaaacgta tataagcgct
      421 gatgttttgc taagtcgagg ttagtatggc ttcatctctc atgagaataa gaacaacaac
      481 aaatagagca agcaaattcg agaattcctg cagcccgggc aattcgaata taatgtctaa
      541 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      601 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
      661 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
      721 ttttacgtat ggtgttcaat gcttttcaag atacccagat catatgaaac ggcatgactt
      781 tttcaagagt gccatg
//
