LOCUS       sequence-94367-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sdChR(C138S) fused to GCaMP6f in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Venkatachalam V, Cohen AE
  TITLE     Imaging GFP-Based Reporters in Neurons with Multiwavelength 
            Optogenetic Control.
  JOURNAL   Biophys J. 2014 Oct 7;107(7):1554-63. doi: 
            10.1016/j.bpj.2014.08.020.
  PUBMED    25296307
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2014.08"; journalName: "Biophys J"; date: "2014-10-7-
            7"; volume: "107"; issue: "7"; pages: "1554-63"
COMMENT     Sequence Label: VV247: sdChR(C138S)-TS-GCaMP6f-ER in fck
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..61)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(89..103,104..112))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(116..148)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(152..169)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgagtgagct cagccgacct atagctctga ctgcgtgacc tgtcttattc cacttacgac
       61 gtgatgagtc gaccatggtg gcgagatcct tatcgtcatc gtcgtacaga tcccgaccca
      121 tttgctgtcc accagtcatg ctagccatac catgatgatg atgatgatga gaacccatca
      181 cgttgatgtc gatctggtcc agggggatgt actcgccctc gctggtgatc ctgctcttgg
      241 cggccgcctt gctcactgct actaccggtg ccgccacttt atcttcctct gtcacgaagg
      301 ccagcacgtc caccttgcgt cccagaaact gggagctcac tggtctcctg atatcgccgt
      361 acataatgat atgctcgtga attttgatcc gcaggaagtg ccccagcagt ccccaaatat
      421 tcttggacag caggtcggcg atggtatggc caatagtgga cccagccacg ctcagggtgt
      481 gcatgccttc aggcccaaag atgaacaggc cgggaaacat cagccaacta gaatagtaca
      541 cggcagtcat ggccagcacc agtttcttac agccgccggc aggcatgata tagtaagact
      601 ccacgtagat gattccagcg ttgtagaatg tctgggttcc atacaccagg ccgatacagt
      661 aaaacagcca cttcacccac ccagtggcca gagcggctgt cactcccatg caaatagttc
      721 ccaggtcgga caccagcaga gccattgtcc gcttattg
//
