LOCUS       sequence-94368-e        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian codon optimized CRY2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Lee S, Park H, Kyung T, Kim NY, Kim S, Kim J, Heo WD
  TITLE     Reversible protein inactivation by optogenetic trapping in cells.
  JOURNAL   Nat Methods. 2014 Jun;11(6):633-6. doi: 10.1038/nmeth.2940. Epub 
            2014 May 4.
  PUBMED    24793453
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2940"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "633-6"
COMMENT     Sequence Label: pCMV-CRY2-mCherry
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..745)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(313..332)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     582..600
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      742..751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagaattcga agcttgagct cgagatctga gtccggactt gtacagctcg tccatgccgc
       61 cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag tcctcgttgt
      121 gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc acgggcttct
      181 tggccttgta ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc agcttcagcc
      241 tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg gaggaggcct
      301 cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca
      361 gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc acggtcacca
      421 cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct
      481 tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg tacatgaact
      541 gaggggacag gatgtcccag gcgaagggca gggggccacc cttggtcacc ttcagcttgg
      601 cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc
      661 cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg atggccatgt
      721 tatcctcctc gcccttgctc accatggtgg cgaccggtgg gctgccgctg ccggcagcac
      781 cgatcataat ctgcgcctcc cgagtgcgag agatcgcttt agccagcagc tcgcgtgctg
      841 tatctatatc aactataggc ttagcgtaat tggtgcccag ctccaccccg cttgccttga
      901 gcactgtcag tggtgcgtcc caaggatggt gaatccactc cgtaggaagt cgggccagct
      961 cggggagcca ctgtcgaata tactctcctt cgggatcata cttagctccc tgcaaggccg
     1021 gattgtc
//
