LOCUS       sequence-94370-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses CKK domain of CAMSAP1 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Hendershott MC, Vale RD
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and 
            Patronin.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5860-5. doi: 
            10.1073/pnas.1404133111. Epub 2014 Mar 26.
  PUBMED    24706919
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404133111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-04-22- 22"; volume: "111"; issue: "16"; pages: "5860-5"
COMMENT     Sequence Label: pET-28a+GFP-CAMSAP1 CKK
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(681..701)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cttgtagcag ccggatctca gtggtggtgg tggtggtgct cgagtgccta tcatttacga
       61 gtctgggcct tctttggcac tgcaggccgc ttgggctgcc acaggtggtt gtggattgtg
      121 agtgcgtcca cactgacaga catggttttg gctgggatca agttaaactg ttttcggtct
      181 gagctgtatt tatacagttt gtcgatcatt ttcttggtga tgttctttgg ccccgtgcca
      241 gtgagtttgt agatttcctc agtatcagga tagtagcagt aaagcgccct gaactggcag
      301 ccagcatcac gaaacagtat gatgtagtga ttggcatcac acttctccag ctcctccaat
      361 atggaattct tgtggggttc gttcactttt ccagccaggc agcaatggga tatggcattg
      421 tgaataatcg gcttgtttga tttactactg ggctccttaa agagcttggg acctgtgtac
      481 tcggccactg aggccaggga agatgccgcc gacgcggtct cccagtctcg gtctgtgctc
      541 ctgcttgggt tacggctcag tatgggcagg gcttccatcg attccactcg ctgagagggt
      601 gtgcccccgg aatgaacgct ctcgggttct gtcgtcgccg cagaggccaa ggacaggctg
      661 gagcctgtac cacctccacc cccctgaaaa tacagatttt cggatccttt gtagagctca
      721 tccatgccat gtgtaat
//
