LOCUS       sequence-94400-e        8330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knocks down endogenous TACC3 and expresses RNAi-resistant human 
            TACC3 tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Cheeseman LP, Harry EF, McAinsh AD, Prior IA, Royle SJ
  TITLE     Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates 
            kinetochore fiber tension.
  JOURNAL   J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. 
            Epub 2013 Mar 26.
  PUBMED    23532825
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2013-05-1"; volume: "126(Pt 9):2102-13. doi"; pages: "
            10.1242/jcs.124834. Epub 2013 Mar 26"
COMMENT     Sequence Label: pBrain-GFP-FKBP-TACC3-shTACC3
FEATURES             Location/Qualifiers
     source          1..8330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     64..86
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     78..95
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     79..95
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     102..121
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        102..120
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     153..169
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     153..169
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        189..403
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     324..343
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(476..492)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(477..493)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(521..539)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(560..576)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(560..576)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(573..595)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    584..600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(608..614,615..632,633..638))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    653..674
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     enhancer        777..1080
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1081..1284
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1235..1255
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1323..1332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1329..1331,1332..1334,1335..2042)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             join(1329..1331,1332..1334,1335..2042)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(1374..1395)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1635..1654)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1982..2003
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(2091..2093,2094..2414)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     polyA_signal    5118..5239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5155..5174)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5209..5228
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5246..5701)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5383..5404)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5595..5614
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5728..5832
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5834..6191
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5834..6025
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5857..5877)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6042..6177
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6226..7020
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6226..7020
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6280..6299)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6890..6909
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7208..7227)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7252..7299
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7628..8216
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8117..8136
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
       61 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
      121 gcgaattgga gctccaccgc ggtggcggcc gctctagaac tagtggatcc cccgggctgc
      181 aggaattcga acgctgacgt catcaacccg ctccaaggaa tcgcgggccc agtgtcacta
      241 ggcgggaaca cccagcgcgc gtgcgccctg gcaggaagat ggctgtgagg gacaggggag
      301 tggcgccctg caatatttgc atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa
      361 tgtctttgga tttgggaatc ttataagttc tgtatgagac cacagatccc cgttaccgga
      421 agatcgtctg ttcaagagac agacgatctt ccggtaactt tttggaaaag cttatcgata
      481 ccgtcgacct cgaggggggg cccggtacca gcttttgttc cctttagtga gggttaattt
      541 cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      601 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      661 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
      721 gccagatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      781 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      841 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      901 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      961 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     1021 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     1081 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     1141 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     1201 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     1261 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
     1321 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1381 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1441 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1501 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     1561 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1621 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1681 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1741 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1801 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     1861 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1921 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     1981 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2041 accgaatcga tgtgaatggg ggccctgaac ccgccaagaa cctgtacacc atgggagtgc
     2101 aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc cagacctgcg
     2161 tggtgcacta caccgggatg cttgaagatg gaaagaaatt tgattcctcc cgggacagaa
     2221 acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg
     2281 ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat tatgcctatg
     2341 gtgccactgg acacccaggc atcatcccac cacatgccac tctcgtcttc gatgtggagc
     2401 ttctaaaact ggaacggtac aagtccggac tcagatctcg agctcaagct tcgaattctg
     2461 cagtcgacgg taccgcgggc ccgggtatga gtctgcaggt cttaaacgac aaaaatgtca
     2521 gcaatgaaaa aaatacagaa aattgcgact tcctgttttc gccaccagaa gttacaggta
     2581 ggtcatctgt tcttcgtgtg tcacagaaag aaaatgtgcc acccaagaac ctggccaaag
     2641 ctatgaaggt gacttttcag acacctctgc gggatccaca gacgcacagg attctaagtc
     2701 ctagcatggc cagcaaactt gaggctcctt tcactcagga tgacaccctt ggactggaaa
     2761 actcacaccc ggtctggaca cagaaagaga accaacagct catcaaggaa gtggatgcca
     2821 aaactactca tggaattcta cagaaaccag tggaggctga caccgacctc ctgggggatg
     2881 caagcccagc ctttgggagt ggcagctcca gcgagtctgg cccaggtgcc ctggctgacc
     2941 tggactgctc aagctcttcc cagagcccag gaagttctga gaaccaaatg gtgtctccag
     3001 gaaaagtgtc tggcagccct gagcaagccg tggaggaaaa ccttagttcc tattccttag
     3061 acagaagagt gacacccgcc tctgagaccc tagaagaccc ttgcaggaca gagtcccagc
     3121 acaaagcgga gactccgcac ggagccgagg aagaatgcaa agcggagact ccgcacggag
     3181 ccgaggagga atgccggcac ggtggggtct gtgctcccgc agcagtggcc acttcgcctc
     3241 ctggtgcaat ccctaaggaa gcctgcggag gagcacccct gcagggtctg cctggcgaag
     3301 ccctgggctg ccctgcgggt gtgggcaccc ccgtgccagc agatggcact cagaccctta
     3361 cctgtgcaca cacctctgct cctgagagca cagccccaac caaccacctg gtggctggca
     3421 gggccatgac cctgagtcct caggaagaag tggctgcagg ccaaatggcc agctcctcga
     3481 ggagcggacc tgtaaaacta gaatttgatg tatctgatgg cgccaccagc aaaagggcac
     3541 ccccaccaag gagactggga gagaggtccg gcctcaagcc tcccttgagg aaagcagcag
     3601 tgaggcagca aaaggccccg caggaggtgg aggaggacga cggtaggagc ggagcaggag
     3661 aggacccccc catgccagct tctcggggct cttaccacct cgactgggac aaaatggatg
     3721 acccaaactt catcccgttc ggaggtgaca ccaagtctgg ttgcagtgag gcccagcccc
     3781 cagaaagccc tgagaccagg ctgggccagc cagcggctga acagttgcat gctgggcctg
     3841 ccacggagga gccaggtccc tgtctgagcc agcagctgca ttcagcctca gcggaggaca
     3901 cgcctgtggt gcagttggca gccgagaccc caacagcaga gagcaaggag agagccttga
     3961 actctgccag cacctcgctt cccacaagct gtccaggcag tgagccagtg cccacccatc
     4021 agcaggggca gcctgccttg gagctgaaag aggagagctt cagagacccc gctgaggttc
     4081 taggcacggg cgcggaggtg gattacctgg agcagtttgg aacttcctcg tttaaggagt
     4141 cggccttgag gaagcagtcc ttatacctca agttcgaccc cctcctgagg gacagtcctg
     4201 gtagaccagt gcccgtggcc accgagacca gcagcatgca cggtgcaaat gagactccct
     4261 caggacgtcc gcgggaagcc aagcttgtgg agttcgattt cttgggagca ctggacattc
     4321 ctgtgccagg cccaccccca ggtgttcccg cgcctggggg cccacccctg tccaccggac
     4381 ctatagtgga cctgctccag tacagccaga aggacctgga tgcagtggta aaggcgacac
     4441 aggaggagaa ccgggagctg aggagcaggt gtgaggagct ccacgggaag aacctggaac
     4501 tggggaagat catggacagg ttcgaagagg ttgtgtacca ggccatggag gaagttcaga
     4561 agcagaagga actttccaaa gctgaaatcc agaaagttct aaaagaaaaa gaccaactta
     4621 ccacagatct gaactccatg gagaagtcct tctccgacct cttcaagcgt tttgagaaac
     4681 agaaagaggt gatcgagggc taccgcaaga acgaagagtc actgaagaag tgcgtggagg
     4741 attacctggc aaggatcacc caggagggcc agaggtacca agccctgaag gcccacgcgg
     4801 aggagaagct gcagctggca aacgaggaga tcgcccaggt ccggagcaag gcccaggcgg
     4861 aagcgttggc cctccaggcc agcctgagga aggagcagat gcgcatccag tcgctggaga
     4921 agacagtgga gcagaagact aaagagaacg aggagctgac caggatctgc gacgacctca
     4981 tctccaagat ggagaagatc tctagataac tgatcataat cagccatacc acatttgtag
     5041 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     5101 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5161 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5221 aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     5281 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     5341 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     5401 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     5461 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     5521 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     5581 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     5641 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     5701 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5761 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5821 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     5881 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5941 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6001 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6061 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6121 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6181 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     6241 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6301 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6361 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     6421 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6481 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6541 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6601 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6661 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6721 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     6781 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6841 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     6901 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     6961 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     7021 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     7081 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     7141 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     7201 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     7261 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     7321 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     7381 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     7441 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     7501 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7561 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7621 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7681 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7741 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     7801 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7861 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7921 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7981 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8041 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8101 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8161 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8221 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8281 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
