LOCUS       sequence-94401-e        6794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded fluorescent voltage indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Mishina Y, Mutoh H, Song C, Knopfel T
  TITLE     Exploration of genetically encoded voltage indicators based on a 
            chimeric voltage sensing domain.
  JOURNAL   Front Mol Neurosci. 2014 Sep 29;7:78. doi: 10.3389/fnmol.2014.00078.
            eCollection 2014.
  PUBMED    25324718
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2014-09-29"; volume: "7:78. doi"; pages: " 
            10.3389/fnmol.2014.00078. eCollection 2014"
COMMENT     Sequence Label: pCAG-Chimeric_Butterfly_CY_1.0
FEATURES             Location/Qualifiers
     source          1..6794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1735..1744
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1741..1743,1744..1746,1747..2457)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3469..3720
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(3818..3837)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3883..3938
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3937..3956)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4297..4313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4297..4313)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4310..4332)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4321..4337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4345..4351,4352..4369,4370..4375))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4390..4411
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4470..4665
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4516..4651
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4671..4805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4708..4727)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4762..4781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4873..4890)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5044..5632)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5124..5143)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5803..6594,6595..6663))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6426..6445
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6664..6768)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcgc tagcgccacc
     1741 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1801 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1861 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1921 ctcgtgacca ccttcggcta cggcctgatg tgcttcgcgc gctaccccga ccacatgaag
     1981 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     2041 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2101 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2161 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     2221 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     2281 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     2341 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     2401 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggag
     2461 gaacgaatag atatacccga gatatcaggt ctatggtggg gcgagaatga acatggagtg
     2521 gacgatggga gaatggagat acctactact ggtgtaggtc gcgtccagtt tcgtgtccga
     2581 gcagtgattg atcatctagg gatgcgagtc tttggagtct tcctaatttt cttggacatc
     2641 atcctcatga tcattgatct cagtcttcca ggaaaaagtg aatcttcaca atccttttat
     2701 gacgggatgg ctttggctct ttcttgttat ttcatgctgg atttaggatt aaggatattt
     2761 gcctacgggc ccaagaattt cttcaccaac ccctgggagg ttgctgatgg tttgattatc
     2821 gttgtcacat tcgtcgtcac gatattttac actgtgttag atgaatacgt gcaagaaaca
     2881 ggagccgatg gtttggggca gttggttgtg ttggcccgtt tgctgcgtat cttcaagctg
     2941 acccgccact tcgtgggcca ccaacaaatg aaggcttcct cgagaagaac aatatcacag
     3001 aacaagcgtc gaaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     3061 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     3121 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     3181 accctcgtga ccaccctgac ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg
     3241 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3301 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3361 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3421 cacaagctgg agtacaacgc catcagcgac aacgtctata tcaccgccga caagcagaag
     3481 aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     3541 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     3601 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
     3661 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     3721 taaaagctta aggcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg
     3781 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
     3841 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     3901 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     3961 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat
     4021 gctggctgcc atgaacaaag gtggctataa agaggtcatc agtatatgaa acagccccct
     4081 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     4141 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     4201 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga
     4261 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     4321 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     4381 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4441 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
     4501 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4561 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     4621 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta
     4681 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4741 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     4801 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4861 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4921 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4981 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5041 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5101 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5161 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5221 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5281 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5341 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5401 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5461 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     5521 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5581 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5641 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5701 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5761 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5821 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5881 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5941 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6001 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6061 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6121 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6181 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6241 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6301 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6361 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6421 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6481 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6541 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6601 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6661 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6721 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6781 aaaagtgcca cctg
//
