LOCUS       sequence-94402-e       10646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses knockdown-proof human CHC17 tagged with GFP.  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Booth DG, Hood FE, Prior IA, Royle SJ
  TITLE     A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by 
            inter-microtubule bridging.
  JOURNAL   EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 
            2011 Feb 4.
  PUBMED    21297582
REFERENCE   2  (bases 1 to 10646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011.15"; journalName: "EMBO J"; date: "2011-03-2- 
            2"; volume: "30"; issue: "5"; pages: "906-19"
COMMENT     Sequence Label: GFP-CHC17KDP
FEATURES             Location/Qualifiers
     source          1..10646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     RBS             4421..4429
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    7434..7555
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7471..7490)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7525..7544
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7562..8017)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7699..7720)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7911..7930
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8044..8148
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8150..8507
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8150..8341
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8173..8193)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8358..8493
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8542..9336
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8542..9336
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8596..8615)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9206..9225
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9524..9543)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9568..9615
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9944..10532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10433..10452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctatggcc cagattctgc caattcgttt tcaggagcat
     1381 ctccagctcc agaacctggg tatcaaccca gcaaacattg gcttcagtac cctgactatg
     1441 gagtctgaca aattcatctg cattagagaa aaagtaggag agcaggccca ggtggtaatc
     1501 attgatatga atgacccaag taatccgatc agacgtccta tttcagcaga cagcgccatc
     1561 atgaatccag ctagcaaagt aattgcactg aaagctggga aaactcttca gatttttaac
     1621 attgaaatga aaagtaaaat gaaggctcat accatgactg atgatgtcac cttttggaaa
     1681 tggatctctt tgaatacggt tgctcttgtt acggataatg cagtttatca ctggagtatg
     1741 gaaggagagt ctcagccagt gaaaatgttt gatcgccatt ctagccttgc agggtgccag
     1801 attatcaatt accgtacaga tgcaaaacaa aagtggttac ttctgactgg tatatctgca
     1861 cagcaaaatc gtgtggtggg agctatgcag ctatattctg tagataggaa agtgtctcag
     1921 cccattgaag gacatgcagc tagctttgca cagtttaaga tggaaggaaa tgcagaagaa
     1981 tcaacgttat tttgttttgc agttcggggc caagctggag ggaagttaca tattattgaa
     2041 gttggcacac cacctacagg gaaccagccc tttccaaaga aggcagtgga tgtcttcttt
     2101 cctccagaag cacaaaatga ttttcctgtt gcaatgcaga tcagtgaaaa gcatgatgtg
     2161 gtgttcttga taaccaagta tggttatatc cacctctatg atcttgagac tggtacctgc
     2221 atctacatga atagaatcag tggagaaaca atttttgtta ctgcacctca tgaagccaca
     2281 gctggaataa ttggagtaaa cagaaaggga caagttctgt cagtgtgtgt ggaagaagaa
     2341 aacataattc cttacatcac caatgttcta caaaatcctg atttggctct gagaatggct
     2401 gtacgtaata acttagccgg tgctgaagaa ctctttgccc ggaaatttaa tgctcttttt
     2461 gcccagggaa attactcgga ggcagcaaag gtggctgcta atgcaccaaa gggaattctt
     2521 cgtactccag acactatccg tcggttccag agtgtcccag cccagccagg tcaaacttct
     2581 cctctacttc agtactttgg tatccttttg gaccagggac agctcaacaa atacgaatcc
     2641 ttagagcttt gtaggcctgt acttcagcaa gggcgaaaac agcttttgga gaaatggtta
     2701 aaagaagata agctggaatg ttctgaagaa ctgggtgatc ttgtgaaatc tgtggaccct
     2761 acattggcac ttagtgtgta cctaagggct aacgtcccaa ataaagtcat tcagtgcttt
     2821 gcagaaacag gtcaagtcca aaagattgtt ttatatgcta aaaaagttgg atacactcca
     2881 gattggatat ttctgctgag aaatgtaatg cgaatcagtc cagatcaggg acagcagttt
     2941 gcccaaatgt tagttcaaga tgaagagcct cttgctgaca tcacacagat tgtagatgtc
     3001 tttatggaat acaatctaat tcagcagtgt actgcattct tgcttgatgc tctgaagaat
     3061 aatcgcccat ctgaaggtcc tttacagacg cggttacttg agatgaacct tatgcatgcg
     3121 cctcaagttg cagatgctat tctaggcaat cagatgttca cacattatga ccgggctcat
     3181 attgctcaac tgtgtgaaaa ggctggccta ctgcagcgtg cattagaaca tttcactgat
     3241 ttatatgata taaaacgtgc agtggttcac acccatcttc ttaaccctga gtggttagtc
     3301 aactactttg gttccttatc agtagaagac tccctagaat gtctcagagc catgctgtct
     3361 gccaacatcc gtcagaatct gcagatttgt gttcaggtgg cttctaaata tcatgaacaa
     3421 ctgtcaactc agtctctgat tgaacttttt gaatctttca agagttttga aggtctcttt
     3481 tattttctgg gatccattgt taactttagc caggacccag atgtgcactt taaatatatt
     3541 caggcagctt gcaagactgg gcaaatcaaa gaagtagaaa gaatctgtag agaaagcaac
     3601 tgctacgatc ctgagcgagt caagaatttt cttaaggaag caaaactaac agatcagcta
     3661 ccacttatca ttgtgtgtga tcgatttgac tttgtccatg atttggtgct ctatttatat
     3721 agaaataatc ttcaaaagta tatagagata tatgtacaga aggtgaatcc aagtcgactt
     3781 cctgtagtta ttggaggatt acttgatgtt gactgttctg aagatgtcat aaaaaacttg
     3841 attcttgttg taagaggtca attctctact gatgagcttg ttgctgaggt tgaaaaaaga
     3901 aacagattga aactgcttct gccttggcta gaggccagaa ttcatgaggg ctgtgaggag
     3961 cctgctactc acaatgcctt agccaaaatc tacatagaca gtaataacaa cccggagaga
     4021 tttcttcgtg aaaatcccta ctatgacagt cgcgttgttg gaaagtattg tgagaagaga
     4081 gatccacatc tggcctgtgt tgcttatgaa cgtggccaat gtgatctgga acttattaat
     4141 gtttgcaatg agaattccct cttcaaaagt ctttctcgct acctggtacg tcgaaaggat
     4201 ccagaattgt ggggcagcgt gctgctggaa agcaatcctt acaggagacc cctaattgac
     4261 caggttgtac aaacagcttt gtctgagact caggaccctg aagaagtgtc agtaactgta
     4321 aaggctttca tgactgcaga ccttcctaat gaactcattg aactgctgga gaaaattgtc
     4381 cttgataact ctgtattcag tgaacacagg aatctgcaaa acctccttat cctcactgca
     4441 attaaggctg accgtacacg tgttatggag tatattaacc gcctggataa ttatgatgcc
     4501 ccagatattg ccaatatcgc catcagcaat gagctgtttg aagaagcatt tgccattttc
     4561 cggaaatttg atgtcaatac ttcagcagtt caggtcttaa ttgagcatat tggaaacttg
     4621 gatcgggcat atgagtttgc tgaacgttgc aatgaacctg cggtctggag tcaacttgca
     4681 aaagcccagt tgcagaaagg aatggtgaaa gaagccattg attcttatat caaagcagat
     4741 gatccttcct cctacatgga agttgttcag gctgccaata ctagtggaaa ctgggaagaa
     4801 ctggtgaagt acttgcagat ggcccgtaag aaggctcgag agtcctatgt ggagacagaa
     4861 ctgatattcg cactggctaa aacaaaccgc cttgcagagt tagaagaatt tatcaatgga
     4921 ccaaataatg ctcatatcca acaagttggt gaccgttgtt atgatgaaaa aatgtatgat
     4981 gctgctaagt tgttgtacaa taatgtttcc aattttggac gtttggcatc taccctggtt
     5041 cacctgggtg aatatcaggc agctgttgat ggggctagga aagctaacag tactcgaaca
     5101 tggaaagagg tctgcttcgc ctgtgtagat gggaaagaat tccgtcttgc tcagatgtgt
     5161 ggacttcata ttgttgtaca tgcagatgaa ttagaagaac ttatcaacta ctatcaggat
     5221 cgtggctatt ttgaagagct gatcaccatg ttggaagcag cactgggact tgagcgagct
     5281 cacatgggaa tgtttactga attagctatt ctatactcta aatttaagcc tcagaaaatg
     5341 agggagcacc tggagctgtt ctggtctaga gtgaatattc ccaaggtgct aagagctgca
     5401 gaacaagctc atctttgggc agaactggtg tttttgtatg acaagtatga agaatatgat
     5461 aatgccataa ttaccatgat gaatcatcca actgatgcct ggaaagaagg gcaattcaaa
     5521 gatatcatta ccaaggttgc caatgtggaa ctatactaca gagcaataca gttctactta
     5581 gaattcaagc ctctgttgtt aaatgatttg ctgatggtgc tgtctccacg gttggatcac
     5641 actcgtgcag tcaattattt cagcaaggtt aaacagctac cactggtgaa accgtatttg
     5701 cgttcagttc agaaccataa caacaaatct gtgaatgaat cattgaacaa tctttttatt
     5761 acagaagaag attatcaggc tctgcgaaca tcaatagatg cttatgacaa ctttgacaat
     5821 atctcgcttg ctcagcgttt ggaaaaacat gaactcattg agttcaggag aattgctgct
     5881 tatctcttca aaggcaacaa tcgctggaaa cagagtgtag agctgtgcaa gaaagacagc
     5941 ctttacaagg atgcaatgca gtatgcttct gaatctaaag atactgaatt ggctgaagaa
     6001 ctcctgcagt ggtttttgca ggaagaaaaa agagagtgct ttggagcttg tctgtttacc
     6061 tgttacgatc ttttaaggcc agatgtcgtc ctagaaactg catggaggca caatatcatg
     6121 gattttgcca tgccctattt catccaggtc atgaaggagt acttgacaaa ggtggataaa
     6181 ttagatgctt cagaatcact gagaaaagaa gaagaacaag ctacagagac acaacccatt
     6241 gtttatggtc agccccagtt gatgctgaca gcaggaccca gtgttgccgt ccctccccag
     6301 gcaccttttg gttatggtta taccgcacca ccgtatggac agccacagcc tggctttggg
     6361 tacagcatgt gagatgaagc gctgatcctg tagtcaccta ttttcgtact gaaacatcgt
     6421 ctttacccac ttctcagttt ataatggggg aaaacaggca acgtgttctt gtaaccttta
     6481 tttcatgaag gacttctttt tgtttctaac tataaacttg gatcacctat gttaaaacct
     6541 tatttcacat tccacatcat tttagaattt attttcgaag gggaatagtt tcaatgtttt
     6601 attcacttgg gctttttttc ttccccctct ttctttaaag aactgctcaa tattcaatct
     6661 gttgtgaaga acctgatttg cactctgtag tgtttaaaga aacaaagaaa ctctaatatt
     6721 gaatctctta aatttagtgt atgtaaacag cttacaaata cgtattgtct aaatgcattt
     6781 aaatctgttt tattcaaaga aaagctaaag caaaaacact ggcatatgac catgcaagac
     6841 tgtcagtgcc aacaaagaca acactaatca gcacatcgta cactggattg cagtgcttcc
     6901 cagattattg aaaaatgtta cagacaactt gcctgatttt taaatgagcg taaaaggccc
     6961 tctaacctat gcaggtttcc ccattatgca tatagaaaat gctagtatgt tttgctcact
     7021 tcatatgtaa caggtgccct tatgttgtgc tgtatcctgt gctttttctg tgggaccatt
     7081 ccattcagga gcaaagagca ccatgattcc aatcttgtgt gtgtttacta acccttccct
     7141 gaggcttgtg tatgttggat attgtggtgt tttagatcac tgagtgtaca gaagagagaa
     7201 attcaaacaa aatattgctg ttcttcagtt ttgtttgtgg aatttgaaat tactcaaatt
     7261 taaaataaat tactggactg tgggcggccg ccaccgcggg cccgggatcc accggatcta
     7321 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     7381 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     7441 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7501 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     7561 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     7621 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     7681 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     7741 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     7801 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     7861 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     7921 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     7981 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     8041 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8101 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     8161 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     8221 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     8281 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     8341 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8401 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     8461 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     8521 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     8581 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     8641 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     8701 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     8761 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     8821 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     8881 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     8941 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     9001 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     9061 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     9121 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     9181 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     9241 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     9301 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     9361 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     9421 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     9481 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     9541 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     9601 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     9661 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     9721 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     9781 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9841 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9901 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9961 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    10021 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    10081 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    10141 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    10201 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    10261 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10321 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10381 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10441 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10501 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10561 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    10621 tggataaccg tattaccgcc atgcat
//
