LOCUS       sequence-94421-e         545 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for producing PhiC31 attB-containing minicircle in vitro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: pBT346_pLambda-attB-pCA-GFP-pA
FEATURES             Location/Qualifiers
     source          1..545
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..22
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(41..165)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    205..326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(216..235)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     270..289
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(493..514)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcgaattaac cctcactaaa gggaacaaaa gctggagctc catagtgact ggatatgttg
       61 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      121 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgtgcggc cgctctagaa
      181 ctagttgggc gcgcccaatt taaataagat acattgatga gtttggacaa accacaacta
      241 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      301 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
      361 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
      421 ctgattatga tcagttatct agaattctta cttgtaaagc tcgtccatgc cgagagtgat
      481 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      541 gctca
//
