LOCUS       sequence-94422-e         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of FRT5-flanked Neo; homologous recombination targeting 
            vector cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: 
            pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        423..527
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(552..572)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 agctcgaaat taaccctcac taaagggaac aaaagctgga gctgatttaa ataggagatc
       61 atgcatccag atctggtgat aggtggcaag tggtattccg taaggatatc gttttcggga
      121 gtagtgcccc aactggggta acctttgagt tctctcagtt gggggcgtag ggtcgccgac
      181 gttttcggga gtagtgcccc aactggggta acctttgagt tctctcagtt gggggcgtag
      241 ggtcgccgac gttttcggga gtagtgcccc aactggggta acctttgagt tctctcagtt
      301 gggggcgtag ggtcgccgac ggatcctttg tcgacgaagt tcctattccg aagttcctat
      361 tcttcaaaag gtataggaac ttcgctagcg attattcagg tggcactttt cggggaaatg
      421 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      481 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
      541 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
      601 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
      661 ctccccagca ggcc
//
