LOCUS       sequence-94425-e         561 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for PhiC31 integrase-mediated integration of pHb9-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: pBT366_pattB-Hb9-GFP-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..561
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    135..359
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     348..365
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(528..549)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg gcccgaagtt
       61 cctatacctt ttgaagaata ggaacttcgg cgcgcccaat ttcgagcccc agctggttct
      121 ttccgcctca gaagccatag agcccaccgc atccccagca tgcctgctat tgtcttccca
      181 atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag
      241 acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag
      301 ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt
      361 cgaggctgat cagcgagctc tagggctagc gggcccccat gggatcctca ggagagcaca
      421 cacttgcagc tcatgcagcc ggggccactc tcatcaggag ggttcagctt agatctgagt
      481 ccggacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
      541 aggaccatgt gatcgcgctt c
//
