LOCUS       sequence-94430-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FAK fret sensor with 10 nm helix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Ritt M, Guan JL, Sivaramakrishnan S
  TITLE     Visualizing and manipulating focal adhesion kinase regulation in 
            live cells.
  JOURNAL   J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 
            10.1074/jbc.M112.421164. Epub 2013 Feb 7.
  PUBMED    23393139
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.421164"; journalName: "J Biol Chem"; date: 
            "2013-03-29- 29"; volume: "288"; issue: "13"; pages: "8875-86"
COMMENT     Sequence Label: pCDNA-FRT-FAK10
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(345..347,348..860)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(387..408)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(648..667)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tagatgaaga agatacttat acaatgggaa gcggaggaag cggagaattc ggaagcggag
       61 gaagcggaga aaacctgtat tttcagggcg gaagcggaga agaggaagag aaaaaaaaac
      121 agcaggaaga ggaagcagaa aggctgaggc gtattcaaga agaaatggaa aaggaaagaa
      181 aaagacgtga agaagatgaa caacgtcgaa gaaaggagga ggaggaaagg cggatgaaac
      241 ttgagatgga agcaaagaga aaacaagaag aagaagagag aaagaaaagg gaagatgatg
      301 aaaaacgcaa gaagaagggc tctggcggct ctggcggcgc gcccgtgagc aagggcgagg
      361 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      421 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      481 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      541 ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      601 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      661 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      721 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca
      781 tcagcgacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
      841 agatccgcca caacatcgag gacggcagcg tgcagctcg
//
