LOCUS       sequence-9444-ex        7396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 7396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pENTR4 TNRC6A
FEATURES             Location/Qualifiers
     source          1..7396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    4686..4697
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(5625..5724)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5836..5855)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5847..6656
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5914..5933)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      6746..7334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7235..7254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgggaac catggatgct
      481 gattctgcct ccagttctga atcagagaga aacatcacta tcatggcttc agggaacaca
      541 ggtggtgaaa aagatggcct tcggaatagc actggacttg gttcccaaaa caagtttgta
      601 gttggtagca gcagcaataa tgtgggccat ggaagtagta ctgggccatg gggtttttcc
      661 catggagcca taataagcac atgtcaggtc tctgtggatg ctcctgaaag caaatctgaa
      721 agtagcaaca atagaatgaa tgcttggggc actgtaagtt cttcatcaaa tggagggtta
      781 aatccaagca ctttgaattc agctagcaac catggtgcct ggccagtatt agagaacaat
      841 ggacttgccc taaaagggcc tgtagggagt ggtagttctg gcattaatat tcagtgcagt
      901 actataggcc agatgcctaa caatcagagt attaactcta aagtgagtgg tggttctacc
      961 catggtacct ggggaagcct tcaggaaact tgtgaatctg aagtaagtgg tacacagaag
     1021 gtttcattca gtggtcaacc tcaaaatatt accactgaaa tgactggacc aaataacact
     1081 actaacttta tgacctctag tttaccaaac tccggttcag tgcagaataa tgagctgcct
     1141 agtagtaaca caggggcctg gcgtgtgagc acaatgaatc atcctcagat gcaggctcca
     1201 tcaggtatga atggcacttc cctttctcac cttagcaatg gagagtcaaa aagtggaggc
     1261 tcttatggta ctacatgggg tgcctatggt tctaattact ctggagacaa atgttcaggc
     1321 cctaatggcc aagctaatgg tgacactgtg aatgcaactc taatgcagcc tggcgtaaat
     1381 ggtcctatgg gcactaactt tcaagttaac acaaacaaag gaggtggtgt gtgggaatct
     1441 ggtgcagcaa actcccagag tacatcatgg ggaagtggaa atggcgcaaa ttctggagga
     1501 agtcgaagag gatggggaac ccctgcacaa aacactggca ctaatttacc cagcgttgag
     1561 tggaacaaac tgcctagcaa tcagcattcc aatgatagtg caaatggcaa tggtaagacg
     1621 tttacaaatg gatggaaatc tactgaggaa gaggatcagg gttctgccac atctcagaca
     1681 aatgagcaaa gcagtgtgtg ggccaaaaca ggaggtacag tggagagcga tggtagtaca
     1741 gaaagcactg gacgccttga ggaaaaagga actggggaaa gtcagagtag agacagaaga
     1801 aaaattgatc agcacacatt actccaaagc attgtaaaca gaactgactt agatccacgt
     1861 gtcctgtcca actctggttg gggacagact cctattaagc agaatactgc ctgggataca
     1921 gaaacatcac ctagagggga acgaaagact gacaatggga cagaggcctg gggaagctct
     1981 gcaacacaga cttttaactc aggggcatgt atagataaga ctagccctaa tggtaatgat
     2041 acctcatctg tatcagggtg gggcgatccc aaacctgctc tgaggtgggg agattccaaa
     2101 ggctcaaact gccagggggg gtgggaagat gattctgctg ctacaggaat ggtcaagagc
     2161 aatcagtggg ggaattgcaa agaggagaag gctgcatgga atgactcgca aaagaataaa
     2221 cagggatggg gtgatggaca aaaatcaagc caagggtggt ctgtttctgc cagtgataac
     2281 tggggagaaa cttcaaggaa taaccattgg ggtgaggcca ataagaaatc cagctcagga
     2341 ggtagtgaca gtgacaggtc cgtttccggt tggaacgaac ttggtaaaac tagttctttc
     2401 acttggggaa acaacataaa tccaaataat tcatcaggat gggatgaatc ttctaaacct
     2461 actccttccc agggatgggg agaccctcca aagtctaatc agtctctagg ttggggagat
     2521 tcgtcaaagc cagtcagctc tccagactgg aacaagcaac aagacattgt tggatcttgg
     2581 ggaatcccac cagctacagg caaacctcct ggtacaggct ggctgggggg acctatacca
     2641 gccccagcaa aagaagaaga acccacaggc tgggaggaac catccccaga atctatacgt
     2701 cgcaaaatgg agattgatga tggaacttca gcttggggag atccaagcaa atacaactac
     2761 aaaaatgtga acatgtggaa caaaaacgtc ccaaatggca acagccgttc agaccagcaa
     2821 gcacaggtac atcagctgct aacgcctgca agtgccatct caaacaaaga ggcaagcagt
     2881 ggctctggct ggggtgagcc ctggggggag ccttctactc cagccacaac tgtggataat
     2941 ggtacttcag catggggtaa gcccatagac agtggtccca gctgggggga acccattgct
     3001 gcggcatcca gcacatccac gtggggctcc agctctgttg gtccacaagc attaagcaaa
     3061 tctgggccaa aatctatgca agatggctgg tgtggtgatg atatgccatt gcctggaaat
     3121 cgccccactg gctgggaaga ggaagaggat gtggagattg gaatgtggaa tagtaattca
     3181 tctcaagagc ttaactcatc tttaaattgg ccaccatata caaagaaaat gtcatcgaag
     3241 ggtctgagtg gcaaaaaaag gagaagggaa aggggaatga tgaaaggtgg aaacaaacaa
     3301 gaagaagcgt ggataaatcc atttgttaaa cagttttcaa acatcagttt ttcgagagac
     3361 tcaccagagg aaaatgtaca aagcaataag atggaccttt ctggaggaat gttacaagac
     3421 aaacgaatgg agatagataa acatagccta aatattggtg attacaatcg aacggtcggg
     3481 aaaggccctg gttctcggcc tcagatttcc aaagagtctt ccatggagcg caatccttat
     3541 tttgataagg atggcattgt agcagatgaa tcccaaaaca tgcagtttat gtccagtcaa
     3601 agcatgaagc ttcccccttc aaatagtgca ctacctaacc aggcccttgg ctccatagca
     3661 gggctgggta tgcaaaactt gaattctgtt agacagaatg gcaatcccag tatgtttggt
     3721 gttggaaaca cagcagcaca accccggggc atgcagcagc ctccagcaca acctcttagt
     3781 tcatctcagc ctaatctccg tgctcaagtg cctcctccat tactctcccc tcaggttcca
     3841 gtttcattgc tgaagtatgc accaaacaac ggtggcctga atccactctt tggccctcaa
     3901 caggtagcca tgctgaacca gctatcccag ctaaaccagc tttctcagat ctcccagtta
     3961 cagcgattgt tagcgcagca gcaaagggcg cagagtcaga gaagcgtgcc ttctgggaac
     4021 cggccgcagc aagaccagca gggtcgacct cttagtgtgc agcagcaaat gatgcaacaa
     4081 tctcgtcaac ttgatccaaa cctgttggtg aagcagcaga ctccaccatc tcagcagcag
     4141 ccactccatc agccagccat gaagtctttc cttgacaatg tcatgcccca cactacacct
     4201 gagctgcaaa aagggccatc accaataaat gctttcagca acttccctat aggcttgaac
     4261 tcaaacttga atgtaaatat ggatatgaac agtattaaag agccacagtc aagactaagg
     4321 aagtggacga cagtggacag catttctgtg aacacatctt tggatcaaaa ctccagcaaa
     4381 catggtgcta tttcaagtgg tttcaggctg gaagagtctc catttgttcc ctatgacttt
     4441 atgaacagca gtacttcacc agccagtcct ccaggttcaa taggagatgg ctggccacgt
     4501 gccaaatcgc ctaacggctc tagcagtgtt aattggccac cagaatttcg tcctggtgag
     4561 ccatggaaag gttatccaaa cattgaccct gaaactgacc cttacgtcac tcctggcagt
     4621 gtcataaaca atctttcaat taatactgtg cgggaagttg accacctcag ggacaggaac
     4681 agtgggtcat cctcatcctt gaacaccacg ctgccttcaa ctagtgcctg gtcatccatt
     4741 cgtgcctcca actacaacgt tcccctcagc agtacagcac aaagcacttc agccagaaat
     4801 agtgattcca aattgacatg gtctcctggt tcagttacaa acacctctct ggctcatgag
     4861 ctgtggaagg tccctttgcc acctaaaaac atcactgctc cgtcccgccc acctccggga
     4921 ctgactggtc agaagccacc cttgtctacg tgggataatt ctccccttcg tataggtgga
     4981 ggatggggaa attctgacgc cagatatacc ccaggttcca gctggggtga gagcagctca
     5041 gggagaataa caaattggct tgttctaaaa aaccttacac ctcagatcga tggctcaact
     5101 ctgcgcactc tgtgcatgca gcacggcccg ctgatcacat tccacctgaa cctccctcac
     5161 ggaaatgctc tggtccgcta cagttcaaaa gaagaggtag tgaaggcaca aaagtctctg
     5221 cacatgtgtg tactggggaa cactactatt cttgctgagt ttgccagtga agaggagatc
     5281 agtcgtttct ttgcacaaag ccagtctctg accccttctc ccggctggca gtctctcggg
     5341 tccagccaga gccggctggg ctccctcgac tgttcccact cattctccag ccggaccgat
     5401 ctcaatcact ggaatggtgc tgggctgtcg ggaactaact gtggagacct tcacggcact
     5461 tcactctggg ggaccccgca ttattccaca agcctgtggg gtcccccaag cagcagcgac
     5521 ccccgaggaa ttagcagccc atctcccatt aacgcttttc tttctgttga ccacctgggt
     5581 gggggtggag agtccatgta agcggccgca ctcgagatat ctagacccag ctttcttgta
     5641 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     5701 cagtcaaaat aaaatcatta tttgccatcc agctgcagct ctggcccgtg tctcaaaatc
     5761 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
     5821 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc
     5881 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     5941 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc
     6001 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     6061 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctggtgatg
     6121 catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc
     6181 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     6241 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     6301 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     6361 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
     6421 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     6481 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     6541 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     6601 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
     6661 aattggttaa ttggttgtaa cattattcag attgggcccc gttccactga gcgtcagacc
     6721 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6781 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6841 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6901 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6961 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7021 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7081 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7141 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7201 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7261 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7321 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7381 cttttgctca catgtt
//
