LOCUS       sequence-9445-ex        7450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 7450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pENTR4 TNRC6B
FEATURES             Location/Qualifiers
     source          1..7450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5679..5778)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5890..5909)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5901..6710
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5968..5987)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      6800..7388
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7289..7308
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc
      481 gacatgagag agaaggagca agaaagggaa gaacagttaa tggaagacaa gaaaaggaag
      541 aaagaggata aaaagaaaaa agaagccact cagaaggtca cggaacaaaa aaccaaagtg
      601 cccgaagtga cgaaaccaag tttaagccaa ccaacggccg ccagcccaat tggcagctct
      661 ccatcgccac cagtcaatgg tggcaacaat gccaaaaggg tggcagtgcc gaacggacaa
      721 ccgccaagcg ccgcccgcta catgcctcgg gaggtgccgc cgcgattccg ttgccagcag
      781 gaccacaaag tgttactaaa acgtgggcag ccccctccac cgtcctgcat gctccttggg
      841 ggtggggcag ggcctcctcc ctgcacagca cctggagcaa acccaaacaa cgcacaagtg
      901 acaggagcgc tgctgcagag tgagagtggg actgcgccag actcaaccct tggaggtgct
      961 gctgcttcaa attatgcaaa ttccacttgg ggctcgggag cctcctccaa caacggcacc
     1021 tcccccaacc caattcacat ctgggacaag gtgattgtag acgggtctga catggaagag
     1081 tggccttgta ttgccagcaa agacactgaa tcttcttccg aaaacaccac cgataacaac
     1141 agtgcctcga accctggctc tgagaagagc actctgccag gaagcaccac tagtaacaaa
     1201 ggaaaaggga gccagtgcca gtctgcaagt tctgggaacg aatgtaatct tggggtctgg
     1261 aaatctgacc ctaaggctaa atctgttcaa tcttccaact ctactacaga gaacaacaat
     1321 ggactaggaa attggaggaa tgtgagtggt caggatagaa ttggacctgg ctctggcttc
     1381 agcaacttta acccaaatag caacccatct gcctggccag cactggtcca agaaggaact
     1441 tctaggaaag gggcattgga aacagataat agtaattcca gtgcacaggt tagcacagta
     1501 ggtcagacat ccagggaaca gcagtcaaag atggaaaatg cgggtgttaa ttttgttgtc
     1561 tctggcagag aacaggctca aattcataac actgatggac caaaaaatgg aaacactaac
     1621 tccttgaact taagttcacc aaaccccatg gagaataagg gaatgccctt tggaatgggc
     1681 ttggggaaca cctccaggag cactgatgcc ccttcacaaa gcactggaga tcgaaagact
     1741 gggagtgttg gatcttgggg tgcagctagg gggccttctg gaactgacac agtctctgga
     1801 caaagcaatt ctggaaacaa tgggaacaat ggaaaagaga gagaggactc ctggaaagga
     1861 gcttctgttc agaaatcaac tgggtcaaaa aatgactctt gggacaacaa taacaggtct
     1921 acgggtgggt cctggaactt tggcccccag gactctaatg acaacaaatg gggtgaaggg
     1981 aacaaaatga catctggggt ctctcaggga gaatggaaac agccgactgg gtctgatgag
     2041 ttgaaaattg gagaatggag tggtccaaac caaccaaatt ctagcactgg agcatgggac
     2101 aatcaaaagg gccaccccct ccctgaaaac caaggcaatg cccaggctcc ctgttgggga
     2161 agatcttcca gctccacagg aagtgaagtt ggaggtcaaa gcactggaag caaccacaaa
     2221 gcaggaagta gtgacagtca taactctggc cgtcggtcgt acaggcccac acatcctgat
     2281 tgtcaggctg tcttgcagac tcttttgagc cgaactgatt tggaccccag ggtgctctca
     2341 aacactggct ggggccaaac tcaaattaag caggacacag tgtgggacat tgaagaggtg
     2401 ccaaggcctg aggggaaatc tgacaaagga actgaggggt gggagagcgc tgccacacag
     2461 accaagaact cagggggctg gggagatgca cccagccaaa gcaatcaaat gaagtctgga
     2521 tggggggagc tctcagcctc tacagagtgg aaagacccca agaacacagg aggctggaat
     2581 gactacaaga acaacaactc ttccaactgg ggaggaggac gacctgatga aaagacacct
     2641 tcctcttgga atgagaatcc cagcaaggat caggggtggg gaggtggacg ccagcccaat
     2701 caaggatggt cttctggaaa gaatggttgg ggggaggaag tcgatcagac aaaaaacagc
     2761 aattgggaaa gttctgcaag taaacctgtg tctgggtggg gtgaaggagg gcagaatgaa
     2821 atcgggactt ggggtaatgg tggcaatgca agcctagctt caaaaggtgg gtgggaggat
     2881 tgcaaaagat ccccagcatg gaatgagacg ggccgacagc ccaattcctg gaataaacaa
     2941 caccaacagc agcagccccc acagcagccg ccgccaccac aaccagaggc ttctggttcg
     3001 tggggaggcc cacccccacc acctccaggc aacgttcgac cttccaattc cagctggagc
     3061 agcgggccac agcctgcaac acctaaggat gaggaaccca gtggttggga agagccatcc
     3121 ccacagtcaa ttagtcggaa aatggacatt gatgatggca cttcagcatg gggagaccct
     3181 aacagttata actacaagaa tgtgaatctg tgggataaga attcccaagg gggcccagca
     3241 cctcgagaac caaacctgcc caccccaatg accagtaaat cggcatcaga ttccaaatct
     3301 atgcaagacg gctgggggga gagtgacggg ccagtcacag gagctcgcca tcccagctgg
     3361 gaagaggagg aggatggagg agtctggaac accactggct ctcagggcag tgcttcctcc
     3421 cacaactcag caagctgggg acaaggagga aagaaacaaa tgaagtgctc actcaaagga
     3481 ggaaacaatg attcatggat gaatcctctt gccaaacagt tttcaaatat gggattgctg
     3541 agtcagactg aagataatcc aagcagcaaa atggatttgt ctgtaggaag cctttcagat
     3601 aaaaaatttg atgtggacaa gcgagcgatg aatctcgggg attttaatga tatcatgagg
     3661 aaggatcgat ctgggttccg tccacctaat tccaaagaca tgggaaccac agatagtggg
     3721 ccttattttg agaagggcgg tagtcatggt ttgtttggaa acagcacagc acaatcgaga
     3781 ggtctgcaca cacccgtgca gccactaaat tcttctccca gtctccgggc gcaagtgcct
     3841 ccccagttta tttcccccca ggtttctgcc tcaatgctca agcagtttcc caacagtggc
     3901 ctgagtccag gtcttttcaa tgtggggccc cagttatctc ctcaacaaat tgccatgctg
     3961 agccagcttc cacaaattcc ccagtttcag ttggcatgtc agcttctctt gcagcagcag
     4021 caacagcagc agttgttaca gaaccagaga aagatttctc aagctgtacg ccaacagcaa
     4081 gagcagcagc tggctcgaat ggtgagtgca ctgcagcagc agcagcagca gcagcagagg
     4141 cagccaggca tgaagcactc gccctctcat cctgttgggc ccaagccgca tctggacaac
     4201 atggtaccca acgcattgaa tgtggggctc ccagaccttc aaaccaaagg gccaatacct
     4261 ggatatggtt ctggcttcag ctctggcggc atggactatg gcatggttgg tgggaaggag
     4321 gctggaaccg agtctcgctt taaacagtgg acctccatga tggaggggct gccctctgta
     4381 gccacacagg aagccaatat gcacaaaaat ggcgctatag tggcccctgg taaaacccgg
     4441 ggagggtcac cgtacaacca gtttgatatc atccctggtg acacactggg tggccatacg
     4501 ggtcctgctg gtgatagctg gttacctgcc aaatctccac caacaaataa aatcggaagt
     4561 aaatccagca atgccagttg gcctccagaa ttccaaccag gagtgccatg gaaaggtatc
     4621 caaaacattg accctgaatc tgacccctat gtcaccccag gaagtgtgct ggggggtaca
     4681 gccacatctc ccattgtaga tactgaccac caactgctgc gggataacac cacagggtct
     4741 aattcttccc tcaacacctc gctgccttca cctggtgcct ggccctacag tgcctctgac
     4801 aactccttta ccaacgttca tagcacttca gcaaagttcc ctgattacaa atcaacatgg
     4861 tccccagatc ccataggaca caaccccact catctctcca acaagatgtg gaaaaaccat
     4921 atttcctcca ggaacactac accgctgccc cgcccacctc ctggtctgac caaccccaaa
     4981 ccatcatctc cctggagcag cacagcaccc cgatcagtca gggggtgggg gacacaggac
     5041 tcacggctcg cctcggcctc tacctggagt gatggtggct cagttcgtcc tagttactgg
     5101 ctggttcttc acaatctcac cccacagatt gatgggtcaa ccttgagaac gatctgcatg
     5161 cagcatggcc cactgctgac attccatctg aatctaaccc agggcactgc cctgatccga
     5221 tacagcacca aacaggaggc ggccaaggcc caaactgcac tgcacatgtg tgtgttggga
     5281 aacactacca tccttgctga gtttgccact gatgatgaag tcagccgctt tctggcacaa
     5341 gctcagcccc ctacacctgc agcaacccca agtgcgccag ctgcggggtg gcagtcgctg
     5401 gagaccggcc agaaccagtc agatcccgtg ggacctgctc tgaatctttt tggtgggtcc
     5461 actgggctcg ggcagtggag cagcagcgct ggtggcagca gcggggccga tcttgctggc
     5521 gcttcattgt gggggccccc aaactattct tctagcttat ggggagtccc aacggtggaa
     5581 gatccccata ggatgggcag ccctgctcct ttactacctg gtgaccttct gggaggaggg
     5641 tcggattcaa tctgagcggc cgcactcgag atatctagac ccagctttct tgtacaaagt
     5701 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     5761 aaataaaatc attatttgcc atccagctgc agctctggcc cgtgtctcaa aatctctgat
     5821 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     5881 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     5941 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     6001 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     6061 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     6121 cggaatttat gcctcttccg accatcaagc attttatccg tactcctggt gatgcatggt
     6181 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     6241 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     6301 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     6361 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     6421 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     6481 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     6541 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     6601 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     6661 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     6721 ttaattggtt gtaacattat tcagattggg ccccgttcca ctgagcgtca gaccccgtag
     6781 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6841 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6901 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6961 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7021 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7081 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7141 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7201 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7261 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7321 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7381 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7441 ctcacatgtt
//
