LOCUS       sequence-94454-e         568 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses 
            tagRFP via P2A cleavage site. SFFV Promoter driven .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951."
COMMENT     Sequence Label: pL-CRISPR.SFFV.tRFP
FEATURES             Location/Qualifiers
     source          1..568
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agttgataac ggactagcct tattttaact tgctatttct agctctaaaa cagagacgta
       61 caaaaaagag caagaagcta aaaaagattt aaaaattatt tttagcgcag ttaatggaac
      121 aggaactaaa tttaccccaa aaatattacg tgaatcagga tataacgtta ttgaggttga
      181 agagcatgca tttgaagatg aaacatttaa aaatgttgta aatccaaatc cagaatttga
      241 tcctgcatga aaaataccgc ttgaatatgg tattaaacat gatgcagata ttattattat
      301 gaatgaccca gatgctgaca gatttggaat ggcaataaaa catgatggtc attttgtaag
      361 attagatgga aatcaaacag gaccaatttt aattgattga aaattatcaa atctaaaacg
      421 cttaaatagc attccaaaaa atccggctct atattcaagt tttgtaacaa gtgatttggg
      481 tgatagaatc gctcatgaaa aatatggagt taatattgta aaaactttaa ctggatttaa
      541 atgaatgggt agagaaattg ctaaagaa
//
