LOCUS       sequence-94457-e         540 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses 
            eGFP via P2A cleavage site. SFFV Promoter driven.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951."
COMMENT     Sequence Label: pL-CRISPR.SFFV.GFP
FEATURES             Location/Qualifiers
     source          1..540
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccgactcggt gccacttttt caagttgata acggactagc cttattttaa cttgctattt
       61 ctagctctaa aacagagacg tacaaaaaag agcaagaagc taaaaaagat ttaaaaatta
      121 tttttagcgc agttaatgga acaggaacta aatttacccc aaaaatatta cgtgaatcag
      181 gatataacgt tattgaggtt gaagagcatg catttgaaga tgaaacattt aaaaatgttg
      241 taaatccaaa tccagaattt gatcctgcat gaaaaatacc gcttgaatat ggtattaaac
      301 atgatgcaga tattattatt atgaatgacc cagatgctga cagatttgga atggcaataa
      361 aacatgatgg tcattttgta agattagatg gaaatcaaac nggaccaatt ttaattgatt
      421 gaaaattatc anatctaaaa cgcttaaata gcattccaaa aaatccggct ctatattcaa
      481 gttttgtaac aagtgatttn ggtgatagaa tcgctcatga aaaatatgga gttaatattg
//
