LOCUS       sequence-9446-ex        7351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 7351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pENTR4 TNRC6C
FEATURES             Location/Qualifiers
     source          1..7351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2763..2780
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(5580..5679)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5791..5810)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5802..6611
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5869..5888)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      6701..7289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7190..7209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc
      481 gacatggcta cagggagtgc ccagggcaac ttcactggac ataccaagaa gacaaatggc
      541 aataatggca ccaatggcgc actcgtccaa agcccttcta atcagagtgc ccttggagca
      601 gggggagcga acagtaatgg aagtgcggcc agagtgtggg gtgtagccac aggctccagc
      661 tctggcctgg ctcactgctc tgtcagtggt ggggatggaa aaatggacac tatgattgga
      721 gatgggagaa gtcagaattg ctggggtgct tccaactcca atgctggcat taatcttaac
      781 cttaatccta atgccaaccc agctgcctgg cctgtacttg gacatgaagg aaccgtggcg
      841 acaggcaacc cttccagtat ttgcagtcca gtcagtgcca taggtcaaaa tatgggcaac
      901 cagaacggga acccaacagg cactttaggt gcttggggaa acttgctgcc acaagagagc
      961 acagaaccac aaacgtccac ttctcagaat gtgtctttca gcgcacaacc tcagaacctt
     1021 aacactgatg gaccaaataa cactaacccc atgaactctt cacccaaccc tatcaatgca
     1081 atgcagacaa atggactgcc aaactggggc atggctgttg gtatgggggc catcatcccg
     1141 ccccacctgc aaggccttcc tggtgctaat ggatcatcag tttctcaagt cagtgggggc
     1201 agtgctgaag gaataagcaa ttctgtgtgg ggactgtccc caggtaaccc tgccacagga
     1261 aatagcaatt ctgggttcag tcaggggaat ggagacactg tgaactcagc attaagtgct
     1321 aaacaaaatg gatccagcag tgctgtgcaa aaggaaggaa gtggaggaaa tgcttgggat
     1381 tcaggacctc ctgctggtcc tggaatactc gcctggggaa ggggcagtgg caacaatggc
     1441 gttggtaata tccattcagg agcttggggc caccccagcc gaagcacctc taacggtgtg
     1501 aatggggaat ggggaaagcc cccaaaccag cattccaaca gtgacatcaa tgggaaagga
     1561 tcaacagggt gggagagtcc tagtgtcacc agccagaacc ctaccgtaca gcctggtggt
     1621 gaacacatga actcctgggc caaagcggca tcttctggaa ctacagcaag tgaaggaagt
     1681 agtgatggtt ctggcaacca caatgaagga agcactggga gggaaggaac gggagaaggc
     1741 cgaaggcgag ataaagggat tatagaccaa gggcacatcc agttgccaag gaatgatctt
     1801 gacccaagag ttctgtctaa tactggttgg ggacagactc ctgtaaagca aaacactgcc
     1861 tgggaatttg aagaatcccc taggtctgaa aggaaaaatg acaatgggac agaggcctgg
     1921 ggttgtgcag ctactcaggc ttcaaactca ggggggaaga acgatgggtc catcatgaac
     1981 agtacaaata cctcttcagt atctgggtgg gtcaacgcgc cacctgccgc tgtgccagca
     2041 aacacaggtt ggggagacag caacaacaaa gcgccaagtg gcccgggggt ttggggggac
     2101 tcgataagct ctactgctgt tagtactgct gctgctgcca agagtggcca tgcttggagt
     2161 ggggccgcaa atcaggagga caagtcaccc acctggggtg agcctccaaa gcccaaatcc
     2221 caacactggg gagatggaca aagatcaaat ccagcctgga gtgcaggagg gggagattgg
     2281 gcagattcat cgtctgtcct tggacacttg ggggatggga aaaaaaatgg atctggatgg
     2341 gatgctgaca gtaataggtc agggtctggt tggaatgaca ccacgagatc tgggaacagt
     2401 ggctggggca acagcacaaa tacaaaggcc aatccaggta caaactgggg ggagacttta
     2461 aaacctggcc cccaacagaa ctgggctagc aaaccccaag acaacaatgt gagtaactgg
     2521 ggaggagctg cttctgtgaa acagacagga acagggtgga tcggggggcc ggtaccggtc
     2581 aaacagaagg acagcagtga agcaactggc tgggaagaac cctctccacc gtccattcgc
     2641 cgcaaaatgg aaattgatga tggtacctca gcttgggggg acccaagcaa ctataacaat
     2701 aaaactgtaa acatgtggga tagaaacaac ccggtcatcc agagcagtac cacgaccaat
     2761 accaccacca ccaccaccac taccacgagc aacaccacac acagggtcga gacgccgccc
     2821 ccgcaccagg ctggtactca gctgaatcga tcaccgttgc ttggtccagg taggaaagtt
     2881 tcatcaggct ggggagaaat gcctaatgtt cactcaaaga ctgaaaactc ttggggagaa
     2941 ccatcctccc cttctaccct ggtggataat ggcacagcag catgggggaa gccacccagc
     3001 agtggcagcg ggtggggaga tcaccctgcc gagccgccgg tggcatttgg aagagctggc
     3061 gcacctgttg ctgcctcagc cctgtgcaaa ccagcttcaa aatctatgca agaaggctgg
     3121 ggcagtggtg gggatgaaat gaacctcagt accagccagt gggaggatga agaaggggac
     3181 gtgtggaata atgctgcttc ccaagaaagc acctcctcct gcagctcctg ggggaacgcc
     3241 cccaaaaaag gacttcaaaa gggcatgaag acgtctggca agcaggatga ggcctggatc
     3301 atgagccggc tgatcaaaca actcacagac atgggcttcc cgagagagcc agctgaggag
     3361 gccttgaaga gtaacaatat gaatcttgat caggccatga gcgctctgct ggaaaagaag
     3421 gtggacgtgg acaagcgtgg gctgggagtg accgaccata atggaatggc cgccaagccc
     3481 ctcggctgcc gcccgccaat ctccaaagag tcttccgtgg accgccccac ctttcttgac
     3541 aaggatggcg gcctcgtgga agagcccacg ccttcaccgt tcttgccttc cccaagcctg
     3601 aagctccccc tttcacacag tgcactcccc agtcaggccc tgggtgggat tgcctccggg
     3661 ctgggcatgc aaaacttgaa ttcttctaga cagataccga gtggcaatct gggtatgttt
     3721 ggcaatagtg gagcagcaca agccaggacc atgcagcagc cgccacagcc accagtgcag
     3781 cctcttaact cttcccagcc cagtctccgt gctcaagtgc ctcagtttct atcccctcag
     3841 gttcaagcac agcttttgca gtttgcagca aaaaacattg gtctcaaccc tgcactatta
     3901 acctcgccaa ttaatcctca acatatgacg atgttgaacc agctctatca gctgcagctg
     3961 gcataccaac gtttacaaat ccagcagcag atgttacagg cccagcgtaa tgtgtccgga
     4021 tccatgagac aacaggagca gcaagttgcg cgcacaatca ctaatctgca gcagcagatc
     4081 cagcagcacc agcgccagct ggcccaggcc ctgctcgtga agcagccacc accgccaccg
     4141 cccccgccgc acctgtctct gcacccctct gcaggcaaat cggccatgga cagcttcccc
     4201 tcgcacccac agactcccgg cctacctgac ctgcagacca aagagcagca gtcttcaccc
     4261 aacacctttg ctccttaccc tctcgctgga ctgaacccaa acatgaatgt caacagcatg
     4321 gacatgaccg gtggcttgtc ggtgaaggac ccatcccagt cccagtcacg cctcccccag
     4381 tggacgcacc ccaactccat ggataacttg cccagtgccg cttcccccct ggagcagaac
     4441 cctagcaagc atggtgctat ccctggaggt ctaagcattg ggcctccagg taagtcctcc
     4501 attgatgact cctatggccg gtacgattta atccagaaca gtgagtcacc agccagtcct
     4561 cccgtagctg ttccccatag ctggtcacgt gccaaatctg acagtgataa aatctcaaat
     4621 ggctctagca tcaactggcc cccagaattc catccgggag ttccatggaa aggactgcag
     4681 aatattgacc ctgagaatga ccctgacgtc actcctggca gtgtccccac tgggcctacc
     4741 atcaacacca ccatccagga tgtcaaccgc tacctcctca agagtggagg taaactgtca
     4801 gacatcaaat cgacgtggtc ctctggccct acctcccaca cgcaagcctc tctgtctcat
     4861 gaactatgga aggtgcccag aaacagtact gcacccacga ggccacctcc agggttaacc
     4921 aatcccaagc cctcctccac ctggggtgcc agccccctcg gctggaccag ctcctactcc
     4981 tcgggttctg cctggagcac cgacacctca ggaagaacca gcagctggct cgttcttcga
     5041 aacctcactc cccagattga tggttctaca ctgcggacat tgtgtttgca acatgggcct
     5101 cttatcacat tccacctgaa tctgactcaa ggcaatgctg tggtccggta cagctccaag
     5161 gaggaggctg ccaaggccca gaagtctctg cacatgtgcg tcctgggaaa cactaccatc
     5221 ctggccgagt tcgctggtga agaagaagtg aatcgcttct tagcccaagg ccaggcgctg
     5281 ccacccactt ccagctggca gtccagcagc gcgtccagcc agccgcggct cagcgcagcg
     5341 ggcagctccc atggcctggt acgcagcgac gctggccact ggaacgcccc gtgcctgggt
     5401 ggcaagggga gcagtgagct gctgtggggc ggggtgcccc agtactccag cagcctgtgg
     5461 ggcccgccca gcgccgacga cagcagggtg ataggcagcc ccacgccgct aaccaccctg
     5521 ctgcctgggg acctgctcag cggggagtcc ctgtagggcg gccgcattct tatatctaga
     5581 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     5641 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc
     5701 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     5761 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     5821 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     5881 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     5941 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     6001 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     6061 gtactcctgg tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     6121 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     6181 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     6241 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     6301 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     6361 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     6421 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     6481 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     6541 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     6601 agtttttcta atcagaattg gttaattggt tgtaacatta ttcagattgg gccccgttcc
     6661 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6721 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6781 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6841 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6901 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6961 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7021 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7081 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7141 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7201 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7261 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7321 tggccttttg ctggcctttt gctcacatgt t
//
