LOCUS       sequence-94460-e         661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses 
            eGFP via P2A cleavage site. SFFV Promoter driven.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951."
COMMENT     Sequence Label: pL-CRISPR.SFFV.GFP
FEATURES             Location/Qualifiers
     source          1..661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     126..145
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa
       61 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
      121 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
      181 tcttgtggaa aggacgaaac accggagacg gttgtaaatg agcacacaaa atacacatgc
      241 taaaatatta tattctatga cctttataaa atcaaccaaa atcttctttt taataacttt
      301 agtatcaata attagaattt ttatgttcct ttttgcaaac ttttaataaa aatgagcaaa
      361 ataaaaaaac gctagtttta gtaactcgcg ttgttttctt cacctttaat aatagctact
      421 ccaccacttg ttcctaagcg gtcagctcct gcttcaatca ttttttgagc atcttcaaat
      481 gttctaactc caccagctgc tttaactaaa gcattgtctt taacaactga cttcattagt
      541 ttaacatctt caaatgttgc acctgatttt gaaaatcctg ttgatgtttt aacaaattct
      601 aatccagctt caacagctat ttcacaagct ttcatgattt cttcttttgt taataaacaa
      661 t
//
