LOCUS       sequence-94462-e         590 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin 
            resistance, EFS Promoter driven.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951."
COMMENT     Sequence Label: pL-CRISPR.EFS.PAC
FEATURES             Location/Qualifiers
     source          1..590
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     127..146
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa
       61 tatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
      121 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
      181 atcttgtgga aaggacgaaa caccggagac ggttgtaaat gagcacacaa aatacacatg
      241 ctaaaatatt atattctatg acctttataa aatcaaccaa aatcttcttt ttaataactt
      301 tagtatcaat aattagaatt tttatgttcc tttttgcaaa cttttaataa aaatgagcaa
      361 aataaaaaaa cgctagtttt agtaactcgc gttgttttct tcacctttaa taatagctac
      421 tccaccactt gttcctaagc ggtcagctcc tgcttcaatc attttttgag catcttcaaa
      481 tgttctaact ccaccagctg ctttaactaa agcattgtct ttaacaactg acttcattag
      541 tttaacatct tcaaatgttg cacctgattt tgaaaatcct gttgatgttt
//
