LOCUS       sequence-94465-e         311 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin 
            resistance, EFS Promoter driven.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951."
COMMENT     Sequence Label: pL-CRISPR.EFS.PAC
FEATURES             Location/Qualifiers
     source          1..311
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atttctagct ctaaaacaga gacgtacaaa aaagagcaag aagctaaaaa agatttaaaa
       61 attattttta gcgcagttaa tggaacagga actaaattta ccccaaaaat attacgtgaa
      121 tcaggatata acgttattga ggttgaagag catgcatttg aagatgaaac atttaaaaat
      181 gttgtaaatc caaatccaga atttgatcct gcatgaaaaa taccgcttga atatggtatt
      241 aaacatgatg cagatattat tattatgaat gacccagatg ctgacagatt tggaatggca
      301 ataaaacatg a
//
