LOCUS       sequence-94471-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FAK fret control sensor without FERM domain with no linker .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Ritt M, Guan JL, Sivaramakrishnan S
  TITLE     Visualizing and manipulating focal adhesion kinase regulation in 
            live cells.
  JOURNAL   J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 
            10.1074/jbc.M112.421164. Epub 2013 Feb 7.
  PUBMED    23393139
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.421164"; journalName: "J Biol Chem"; date: 
            "2013-03-29- 29"; volume: "288"; issue: "13"; pages: "8875-86"
COMMENT     Sequence Label: pCDNA-FRT-FAK-kinase0
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(35..56)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(296..315)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             455..706
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     643..664
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
       61 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      121 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      181 cctgacctgg ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
      241 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      301 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      361 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      421 caacgccatc agcgacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
      481 caacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      541 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
      601 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      661 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagttaa ttaagggaag
      721 cggaggaagc ggaggaagcg gaggaagcgg accatcaacc agagattatg aaattcaaag
      781 ggagagaatt gaactggggc gctgcattgg tgaaggacag tttggagatg tgcaccaagg
      841 aatttacatg agtccggaaa atccagctat ggctgtagca atcaaaacat gt
//
