LOCUS       sequence-94562-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector for FAK fret sensor with 20 nm helix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Ritt M, Guan JL, Sivaramakrishnan S
  TITLE     Visualizing and manipulating focal adhesion kinase regulation in 
            live cells.
  JOURNAL   J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 
            10.1074/jbc.M112.421164. Epub 2013 Feb 7.
  PUBMED    23393139
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.421164"; journalName: "J Biol Chem"; date: 
            "2013-03-29- 29"; volume: "288"; issue: "13"; pages: "8875-86"
COMMENT     Sequence Label: pCS2-FAK20
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(651..902)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(693..714)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tctttttcta gcacttcata gttggatttc ttctctaatg cattgcctct catctctccg
       61 taggatctcc tgatttcaag gcaacctagt ttcaaagcaa tttcctggtc cacttgatct
      121 gctatttcta acatatagtc atttttcacc tgctgataga aaaagtttaa agttggtttg
      181 tcctcagtga actggtttag aaatcctttg ggcaggtacc gaattctcag ttcatatttc
      241 cattcttctg gaggatgtgc tagttcaaat ttctctctca cattggatac ccccatgtcc
      301 aggtgcagcc agtgaacctc ctcagactgc agatgactga gtcgcaaccc atagcaggcc
      361 acatttttca ctttgtgaca gtccacaatc ttctgtatta tgcctcgaac atcagtagca
      421 tctccatgcc ggataatgct ggcccacgtc gttggctcgc tgctgttttc aaagtagtga
      481 aaaaccttta ggactcgctc catggccccc ggggaacgct ccatcccagt accgaggtgc
      541 gtctttgcac ttgaacttgg tgtatgattc aagtttggat caaggtaagc tgctgccatt
      601 ccgcttcctc cgcttcctcc gcttcctccg cttcctccgc ttcctctaga cttgtacagc
      661 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
      721 cgcttctcgt tggggtcttt gctcagtttg gactggtagc tcaggtagtg gttgtcgggc
      781 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
      841 ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg
      901 tc
//
