LOCUS       sequence-94577-e         521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of FRT5-flanked Neo; homologous recombination targeting 
            vector cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: 
            pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(213..232)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    257..304
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
       61 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
      121 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
      181 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
      241 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
      301 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
      361 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
      421 gaaggnccag ggctcgcagc caacgtcngg gcggcaggcc ctgcnatagc ctcangttac
      481 tcaggatcat ctntagcctg caggngtgcc accatgcctg g
//
