LOCUS       sequence-94580-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression vector encoding an empty IRES-Puromycin 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Giannou AD, Marazioti A, Spella M, Kanellakis NI, Apostolopoulou H, 
            Psallidas I, Prijovich ZM, Vreka M, Zazara DE, Lilis I, 
            Papaleonidopoulos V, Kairi CA, Patmanidi AL, Giopanou I, Spiropoulou
            N, Harokopos V, Aidinis V, Spyratos D, Teliousi S, Papadaki H, 
            Taraviras S, Snyder LA, Eickelberg O, Kardamakis D, Iwakura Y, 
            Feyerabend TB, Rodewald HR, Kalomenidis I, Blackwell TS, Agalioti T,
            Stathopoulos GT
  TITLE     Mast cells mediate malignant pleural effusion formation.
  JOURNAL   J Clin Invest. 2015 Jun;125(6):2317-34. doi: 10.1172/JCI79840. Epub 
            2015 Apr 27.
  PUBMED    25915587
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI79840"; 
            journalName: "J Clin Invest"; date: "2015-06"; volume: "125"; issue:
            "6"; pages: "2317-34"
COMMENT     Sequence Label: retro-puro vector
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     173..195
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     211..227
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(433..450)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     660..679
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag
       61 gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc
      121 tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa
      181 cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc
      241 gccggaatta gatctctcga ggttaacgaa ttccgccccc cccccctaac gttactggcc
      301 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
      361 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
      421 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
      481 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
      541 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
      601 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
      661 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
      721 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
      781 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaa
//
