LOCUS       sequence-94601-e         564 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to 
            accept gRNA from pUC gRNA Shuttle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA
  TITLE     Targeted genome modifications in soybean with CRISPR/Cas9.
  JOURNAL   BMC Biotechnol. 2015 Mar 12;15:16. doi: 10.1186/s12896-015-0131-2.
  PUBMED    25879861
REFERENCE   2  (bases 1 to 564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2015-03-12"; volume: "15:16. doi"; pages: " 
            10.1186/s12896-015-0131-2"
COMMENT     Sequence Label: p201B Cas9
FEATURES             Location/Qualifiers
     source          1..564
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..24)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggacgttatc actcttccct ctatttttat cggatcttgt caacacttta ttatcaatag
       61 aatcatcttt gagaaaagac tggggcacga tatgatccac gtcgtagtcg gagagccgat
      121 tgatgtccag ttcctgatcc acgtacatgt ccctgccgtt ctgcaggtag tacaggtaga
      181 gcttctcatt ctgaagctgg gtgttttcaa ctgggtgttc cttaaggatt tgggacccca
      241 gttcttttat accctcttca atcctcttca tcctttccct actgttcttc tgtcccttct
      301 gggtagtttg gttctctcgg gccatctcga taacgatatt ctcgggctta tgccttccca
      361 ttactttgac gagttcatcc acgaccttaa cggtctgcag tattcccttt ttgatagctg
      421 ggctacctgc aagattancg atgtgctcgt gaagactgtc cccctggcca gaaacttgtg
      481 ctttctggat gtcctcctta aaggtgagag agtcatcatg gatcaactgc atgaagttcc
      541 ggttggcaaa tccatcggac ttaa
//
