LOCUS       sequence-94604-e         416 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to
            accept gRNA from pUC gRNA Shuttle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA
  TITLE     Targeted genome modifications in soybean with CRISPR/Cas9.
  JOURNAL   BMC Biotechnol. 2015 Mar 12;15:16. doi: 10.1186/s12896-015-0131-2.
  PUBMED    25879861
REFERENCE   2  (bases 1 to 416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2015-03-12"; volume: "15:16. doi"; pages: " 
            10.1186/s12896-015-0131-2"
COMMENT     Sequence Label: p201N Cas9
FEATURES             Location/Qualifiers
     source          1..416
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..47)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tttcttgaca acttcttctg aggggacgtt atcactcttc cctctatttt tatcggatct
       61 tgtcaacact ttattatcaa tagaatcatc tttgagaaaa gactggggca cgatatgatc
      121 cacgtcgtag tcggagagcc gattgatgtc cagttcctga tccacgtaca tgtccctgcc
      181 gttctgcagg tagtacaggt agagcttctc attctgaagc tgggtgtttt caactgggtg
      241 ttccttaagg atttgggacc ccagttcttt tataccctct tcaatcctct tcatcctttc
      301 cctactgttc ttctgtccct tctgggnagt ttggttctct cgggccatct cgataacgat
      361 attctcgggc ttatgccttc ccattacttt gacgagttca tccacgacct taacgg
//
