LOCUS       sequence-94605-e         589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to 
            accept gRNA from pUC gRNA Shuttle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA
  TITLE     Targeted genome modifications in soybean with CRISPR/Cas9.
  JOURNAL   BMC Biotechnol. 2015 Mar 12;15:16. doi: 10.1186/s12896-015-0131-2.
  PUBMED    25879861
REFERENCE   2  (bases 1 to 589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2015-03-12"; volume: "15:16. doi"; pages: " 
            10.1186/s12896-015-0131-2"
COMMENT     Sequence Label: p201G Cas9
FEATURES             Location/Qualifiers
     source          1..589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..46)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttcttgacaa cttcttctga ggggacgtta tcactcttcc ctctattttt atcggatctt
       61 gtcaacactt tattatcaat agaatcatct ttgagaaaag actggggcac gatatgatcc
      121 acgtcgtagt cggagagccg attgatgtcc agttcctgat ccacgtacat gtccctgccg
      181 ttctgcaggt agtacaggta gagcttctca ttctgaagct gggtgttttc aactgggtgt
      241 tccttaagga tttgggaccc cagttctttt ataccctctt caatcctctt catcctttcc
      301 ctactgttct tctgtccctt ctgggtagtt tggttctctc gggccatctc gataacgata
      361 ttctcgggct tatgccttcc cattactttg acgagttcat ccacgacctt aacggtctgc
      421 agtattccct ttttgatagc tgggctacct gcaagattag cgatgtgctc gtgaagactg
      481 tccccctggc cagaaacttg tgctttctgg atgtcctcct taaaggtgag agagtcatca
      541 tggatcaact gcatgaagtt ccggttggca aatccatcgg acttaagaa
//
