LOCUS       sequence-94608-e         569 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to 
            accept gRNA from pUC gRNA Shuttle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA
  TITLE     Targeted genome modifications in soybean with CRISPR/Cas9.
  JOURNAL   BMC Biotechnol. 2015 Mar 12;15:16. doi: 10.1186/s12896-015-0131-2.
  PUBMED    25879861
REFERENCE   2  (bases 1 to 569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2015-03-12"; volume: "15:16. doi"; pages: " 
            10.1186/s12896-015-0131-2"
COMMENT     Sequence Label: p201G Cas9
FEATURES             Location/Qualifiers
     source          1..569
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(269..289)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa
       61 cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca
      121 acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt
      181 attgccaaat gtttgaacga tcggggaaat tcgagctcgg ccggcctcga gacgtcgacg
      241 cgtaagcttc tgcagatatc cgcggtcaca ccttcctctt cttcttgggg tcagccctgc
      301 tgtctccacc gagctgagag aggtcgattc ttgtttcata gagccccgta attgactgat
      361 gaatcagtgt ggcgtccagg acctcctttg tagaggtgta ccgctttctg tctatggtgg
      421 tgtcgaagta cttgaaggct gcaggcgcgc ccaagttggt cagagtaaac aagtggataa
      481 tgttttctgc ctgctccctg atgggcttat ccctgtgctt attgtaagca naaagcacct
      541 tatcgaggtt agcgtcggcg aggatcact
//
