LOCUS       sequence-94609-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to
            accept gRNA from pUC gRNA Shuttle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA
  TITLE     Targeted genome modifications in soybean with CRISPR/Cas9.
  JOURNAL   BMC Biotechnol. 2015 Mar 12;15:16. doi: 10.1186/s12896-015-0131-2.
  PUBMED    25879861
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2015-03-12"; volume: "15:16. doi"; pages: " 
            10.1186/s12896-015-0131-2"
COMMENT     Sequence Label: p201N Cas9
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tctcttaagg tagccaaaat cgatatctag ggataacagg gtaatcgatc actagaattc
       61 ggcgcgccgg gcccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag
      121 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa
      181 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg
      241 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct
      301 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
      361 acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact
      421 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact
      481 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
      541 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa
      601 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
      661 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
      721 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
//
