LOCUS       sequence-94656-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: BAGE_pCSdest
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      187..196
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(197..221)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(275..293)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(276..293)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctagaaga tgaagcacag agctgtgccg tcttcaggct ccaacctcca gctcaccaca
      121 ggggactcct ccttcatcag cctggcttgg agcagctggg cagacaatgc cagaaaaacc
      181 gctctggccg ccatggtgcc tgcttttttg tacaaacttg tgatccttga attcgaatcg
      241 atgggatcct gcaaaaagaa caagtagctt gtattctata gtgtcaccta aatcaagctt
      301 gctccgacgt ccccaggcag aatggcggtt ccctaaacga gcattgctta tatagacctc
      361 ccattaggca cgcctaccgc ccatttacgt caatggaacg cccatttgcg tcattgcccc
      421 tccccattga cgtcaatggg gatgtacttg gcagccatcg cgggccattt accgccattg
      481 acgtcaatgg gagtactgcc aatgtaccct ggcgtacttc caatagtaat gtacttgcca
      541 agttactatt aatagatatt gatgtactgc caagtgggcc atttaccgtc attgacgtca
      601 atagggggcg tgagaacgga tatgaatggg caatgagcca tcccattgac gtcaatggtg
      661 ggtggtccta ttgacgtcaa tgggcattga gccaggcggg ccatttaccg taattgacgt
      721 caatggggga ggcgccatat acgtcaatag gaccgcccat atgacgtcaa taggaaagac
      781 catatataga gaccattgac gtcaatgggg gagtggctat gggcggtatt aggaagcccc
      841 atatatggta tatgggaccg cccattggga ggggctatct acgtcaatag gaaaacccat
      901 atatggaata ctatatggc
//
