LOCUS       sequence-94671-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: SAMSN1_pCSdest
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..69
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     51..68
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    123..147
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga
       61 cactatagaa tacaagctac ttgttctttt tgcaggatcc catcgattcg aattcaagga
      121 tcacaagttt gtacaaaaaa gcaggcacca tgctcaagag aaagccatcc aatgtttcag
      181 agaaggagaa acatcaaaaa ccaaagcgaa gcagcagttt tgggaatttc gatcgttttc
      241 ggaataattc tttatcaaaa ccagatgatt caactgaggc acatgaagga gatcccacaa
      301 atggaagtgg agaacaaagt aaaacttcaa ataatggagg cggtttgggt aaaaaaatga
      361 gagctatttc atggacaatg aagaaaaaag tgggtaaaaa gtacatcaaa gccctttctg
      421 aggaaaagga tgaggaagat ggagagaatg cccacccata tagaaacagt gaccctgtga
      481 ttgggaccca cacagagaag gtgtccctca aagccagtga ctccatggat agtctctaca
      541 gtggacagag ctcatcaagt ggcataacaa gctgttcaga tggtacaagt aaccgggaca
      601 gctttcgact ggatgacgat ggcccctatt caggaccatt ctgtggccgt gccagagtgc
      661 atacggattt cacgccaagt ccctatgaca ctgactccct caaaatcaag aaaggagaca
      721 tcatagacat tatttgcaaa acaccaatgg ggatgtggac aggaatgttg aacaataaag
      781 tgggaaactt caaattcatt tatgtggatg tcatctcaga agaggaagca g
//
