LOCUS       sequence-94672-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: CHODL_pCSdest
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatgagccg cgtggtctcg ctgctgctgg
      181 gcgccgcgct gctctgcggc cacggagcct tctgccgccg cgtggtcagc ggccaaaagg
      241 tgtgttttgc tgacttcaag catccctgct acaaaatggc ctacttccat gaactgtcca
      301 gccgagtgag ctttcaggag gcacgcctgg cttgtgagag tgagggagga gtcctcctca
      361 gccttgagaa tgaagcagaa cagaagttaa tagagagcat gttgcaaaac ctgacaaaac
      421 ccgggacagg gatttctgat ggtgatttct ggatagggct ttggaggaat ggagatgggc
      481 aaacatctgg tgcctgccca gatctctacc agtggtctga tggaagcaat tcccagtacc
      541 gaaactggta cacagatgaa ccttcctgcg gaagtgaaaa gtgtgttgtg atgtatcacc
      601 aaccaactgc caatcctggc cttgggggtc cctaccttta ccagtggaat gatgacaggt
      661 gtaacatgaa gcacaattat atttgcaagt atgaaccaga gattaatcca acagcccctg
      721 tagaaaagcc ttatcttaca aatcaaccag gagacaccca tcagaatgtg gttgttactg
      781 aagcaggtat aattcccaat ctaatttatg ttgttatacc aacaataccc ctgctcttac
      841 tgatactggt tgcttttgga acctgttgtt tccagatgct gcat
//
