LOCUS       sequence-94684-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: SCAF4_pCSdest
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatggacgc cgtcaacgcc ttcaaccagg
      181 agctcttttc gcttatggat atgaaacctc ccatctctag agccaagatg attctcatca
      241 ctaaagctgc tattaaagct attaagcttt ataagcatgt agttcaaata gtagaaaagt
      301 tcatcaaaaa gtgtaaacca gaatacaagg ttccgggatt atatgtaatt gactcaattg
      361 tgcgacagtc tcgtcatcag tttggaactg ataaagatgt ttttgggcca agattctcta
      421 aaaacataac tgccacattc caatatttat atctttgtcc atctgaagat aagagtaaaa
      481 tagttcgtgt gctgaacctt tggcaaaaaa atggagtgtt caaaattgaa attattcaac
      541 ctcttttgga catggcagcg ggaaccagta atgcagcccc agtagcagaa aatgttacca
      601 ataatgaagg ctcacctcca cctccagtaa aagtttcttc tgaacctccc acacaagcca
      661 ctccaaactc cgtcccagct gtaccacagt tgcccagctc tgatgctttt gctgctgtgg
      721 ctcagctgtt tcagacaact caaggccaac agcttcagca gatccttcag acttttcaac
      781 agcctccaaa accacagtct cctgcccttg acaatgctgt gatggctca
//
